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2.3 A structure of the ATP-dependent chromatin remodeler Chd1 bound to the nucleosome in a nucleotide-free state

ABSTRACT:

SUBMITTER: Jean-Paul Armache

PROVIDER: EMPIAR-10876 | biostudies-other |

REPOSITORIES: biostudies-other

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Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state.

Nodelman Ilana M IM Das Sayan S Faustino Anneliese M AM Fried Stephen D SD Bowman Gregory D GD Armache Jean-Paul JP

Nature structural & molecular biology 20220216 2

Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. Here we report a complex of the Chd1 remodeler bound to a nucleosome in a nucleotide-free state, determined by cryo-EM to 2.3 Å resolution. The remodeler stimulates the nucleosome to absorb an additional nucleotide on each strand at two different locations: on the tracking strand within the ATPase binding site and on the guide strand one helical turn from the ATPase motor. Remarkably, the ad ...[more]

PMID: 35173352

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