Dataset Information
Tilt series of cell-cell contact of two PTK-1 cells
Ontology highlight
ABSTRACT:
SUBMITTER: Niels Volkmann
PROVIDER: EMPIAR-11114 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Morphological control enables nanometer-scale dissection of cell-cell signaling complexes.
Nature communications 20221220 1
Protein micropatterning enables robust control of cell positioning on electron-microscopy substrates for cryogenic electron tomography (cryo-ET). However, the combination of regulated cell boundaries and the underlying electron-microscopy substrate (EM-grids) provides a poorly understood microenvironment for cell biology. Because substrate stiffness and morphology affect cellular behavior, we devised protocols to characterize the nanometer-scale details of the protein micropatterns on EM-grids b ...[more]