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CryoEM micrographs of mouse apoferritin in a range of ice thicknesses on different microscope setups

ABSTRACT:

SUBMITTER: Eugene YD Chua

PROVIDER: EMPIAR-11397 | biostudies-other |

REPOSITORIES: biostudies-other

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Measuring the effects of ice thickness on resolution in single particle cryo-EM.

Neselu Kasahun K Wang Bing B Rice William J WJ Potter Clinton S CS Carragher Bridget B Chua Eugene Y D EYD

Journal of structural biology: X 20230124

Ice thickness is a critical parameter in single particle cryo-EM - too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high resolution reconstructions. Here we present the practical effects of ice thickness on resolution, and the influence of energy filters, accelerating voltage, or detector mode. We collected apoferritin data with a wide range of ice thicknesses on three microscope ...[more]

PMID: 36742017

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