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CryoEM micrographs of mouse apoferritin in a range of ice thicknesses on different microscope setups


ABSTRACT:

SUBMITTER: Eugene YD Chua 

PROVIDER: EMPIAR-11397 | biostudies-other |

REPOSITORIES: biostudies-other

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Measuring the effects of ice thickness on resolution in single particle cryo-EM.

Neselu Kasahun K   Wang Bing B   Rice William J WJ   Potter Clinton S CS   Carragher Bridget B   Chua Eugene Y D EYD  

Journal of structural biology: X 20230124


Ice thickness is a critical parameter in single particle cryo-EM - too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high resolution reconstructions. Here we present the practical effects of ice thickness on resolution, and the influence of energy filters, accelerating voltage, or detector mode. We collected apoferritin data with a wide range of ice thicknesses on three microscope  ...[more]

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