Dataset Information
Mouse heavy chain apoferritin in plunge-frozen vitreous ice
Ontology highlight
ABSTRACT:
SUBMITTER: Ulrich Lorenz
PROVIDER: EMPIAR-11212 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach.
Frontiers in molecular biosciences 20221110
We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow dynamics of the embedded particles to occur. Once the laser beam is switched off, the sample revitrifies within just a few microseconds, trapping the particles in their transient configurations, which can subsequently be imaged to obtain a snap shot of the dynamics at this point in time. While we ha ...[more]