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Single particle reconstruction of the human LINE-1 ORF2p without substrate (apo)

ABSTRACT:

SUBMITTER: Trevor Peter van Eeuwen

PROVIDER: EMPIAR-11556 | biostudies-other |

REPOSITORIES: biostudies-other

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Structures, functions and adaptations of the human LINE-1 ORF2 protein.

Baldwin Eric T ET van Eeuwen Trevor T Hoyos David D Zalevsky Arthur A Tchesnokov Egor P EP Sánchez Roberto R Miller Bryant D BD Di Stefano Luciano H LH Ruiz Francesc Xavier FX Hancock Matthew M Işik Esin E Mendez-Dorantes Carlos C Walpole Thomas T Nichols Charles C Wan Paul P Riento Kirsi K Halls-Kass Rowan R Augustin Martin M Lammens Alfred A Jestel Anja A Upla Paula P Xibinaku Kera K Congreve Samantha S Hennink Maximiliaan M Rogala Kacper B KB Schneider Anna M AM Fairman Jennifer E JE Christensen Shawn M SM Desrosiers Brian B Bisacchi Gregory S GS Saunders Oliver L OL Hafeez Nafeeza N Miao Wenyan W Kapeller Rosana R Zaller Dennis M DM Sali Andrej A Weichenrieder Oliver O Burns Kathleen H KH Götte Matthias M Rout Michael P MP Arnold Eddy E Greenbaum Benjamin D BD Romero Donna L DL LaCava John J Taylor Martin S MS

Nature 20231214 7997

The LINE-1 (L1) retrotransposon is an ancient genetic parasite that has written around one-third of the human genome through a 'copy and paste' mechanism catalysed by its multifunctional enzyme, open reading frame 2 protein (ORF2p)1. ORF2p reverse transcriptase (RT) and endonuclease activities have been implicated in the pathophysiology of cancer2,3, autoimmunity4,5 and ageing6,7, making ORF2p a potential therapeutic target. However, a lack of structur ...[more]

PMID: 38096902

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