AP2 in the presence of heparin
Ontology highlight
ABSTRACT:
SUBMITTER: Richard Wayne Baker
PROVIDER: EMPIAR-11604 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization.
Partlow Edward A EA Cannon Kevin S KS Hollopeter Gunther G Baker Richard W RW
Nature structural & molecular biology 20220328 4
Clathrin-mediated endocytosis (CME) is the main route of internalization from the plasma membrane. It is known that the heterotetrameric AP2 clathrin adaptor must open to simultaneously engage membrane and endocytic cargo, yet it is unclear how transmembrane cargos are captured to catalyze CME. Using cryogenic-electron microscopy, we discover a new way in which mouse AP2 can reorganize to expose membrane- and cargo-binding pockets, which is not observed in clathrin-coated structures. Instead, it ...[more]
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