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Enhanced plasma persistence of therapeutic enzymes by coupling to soluble dextran.


ABSTRACT: Conjugation of carboxypeptidase G and arginase, two enzymes of therapeutic interest, to a soluble dextran significantly enhanced plasma persistence in normal and tumour-bearing mice. A prolonged decrease in arginine concentrations in plasma of tumour-bearing mice was demonstrated by using the dextran-linked arginase. Gel filtration of dextran-enzyme conjugate showed that enzyme activity co-chromatographed as a single peak with carbohydrate, and enzyme was shown to be covalently linked to the dextran.

SUBMITTER: Sherwood RF 

PROVIDER: S-EPMC1164813 | biostudies-other | 1977 May

REPOSITORIES: biostudies-other

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