Unknown

Dataset Information

0

The metabolism of potassium dodecyl [35-S]sulphate in the rat.


ABSTRACT: The metabolic fate of potassium dodecyl [(35)S]sulphate was studied in rats. Intraperitoneal and oral administration of the ester into free-ranging animals were followed by the excretion of the bulk of the radioactivity in the urine within 12hr., approximately 17% being eliminated as inorganic [(35)S]sulphate. Similar results were obtained in experiments in which potassium dodecyl [(35)S]sulphate was injected intravenously into anaesthetized rats with bile-duct and ureter cannulae. Analysis of urinary radioactivity revealed the presence of a new ester sulphate (metabolite A). This metabolite was isolated, purified and subsequently identified as the sulphate ester of 4-hydroxybutyric acid by paper, thin-layer and gas chromatography, by paper electrophoresis and by comparison of its properties with those of authentic butyric acid 4-sulphate. The identity of the metabolite was confirmed by isotope-dilution experiments. When either purified metabolite A or authentic potassium butyric acid 4[(35)S]-sulphate was administered to free-ranging rats the bulk of the radioactivity was eliminated unchanged in the urine within 12hr., approx. 20% of the dose appearing as inorganic [(35)S]sulphate. Whole-body radioautography and isolated-liver-perfusion experiments implicated the liver as the major site of metabolism of potassium dodecyl [(35)S]sulphate. It is suggested that butyric acid 4-sulphate probably arises by omega-oxidation of dodecyl sulphate to a fatty acid-like compound, which is then degraded by beta-oxidation.

SUBMITTER: Denner WH 

PROVIDER: S-EPMC1187492 | biostudies-other | 1969 Jan

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1176931 | biostudies-other
| S-EPMC1178763 | biostudies-other
| S-EPMC5546651 | biostudies-literature
| S-EPMC2956967 | biostudies-literature
| S-EPMC4029809 | biostudies-literature
| S-EPMC1173770 | biostudies-other
| S-EPMC1172562 | biostudies-other
| S-EPMC8307572 | biostudies-literature
| S-EPMC1187034 | biostudies-other
| S-EPMC1144966 | biostudies-other