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Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes.

ABSTRACT: We report the construction of a physical map of the Mycoplasma gallisepticum S6 genome by field-inversion gel electrophoresis of DNA fragments generated by digestion of genomic DNA with rare-cutting restriction endonucleases. The size of the M. gallisepticum S6 genome was calculated to be approximately 1,054 kb. The loci of several genes have been assigned to the map by Southern hybridization utilizing specific gene probes.

SUBMITTER: Gorton TS

PROVIDER: S-EPMC176583 | biostudies-other | 1995 Jan

REPOSITORIES: biostudies-other

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Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes.

Gorton T S TS Goh M S MS Geary S J SJ

Journal of bacteriology 19950101 1

We report the construction of a physical map of the Mycoplasma gallisepticum S6 genome by field-inversion gel electrophoresis of DNA fragments generated by digestion of genomic DNA with rare-cutting restriction endonucleases. The size of the M. gallisepticum S6 genome was calculated to be approximately 1,054 kb. The loci of several genes have been assigned to the map by Southern hybridization utilizing specific gene probes. ...[more]

PMID: 7798142

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Mycoplasma gallisepticum S6 - Transcriptome analysis reveals novel regulatory mechanisms in a genome-reduced bacterium

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