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A protein binding assay for adenosine 3':5'-cyclic monophosphate.

ABSTRACT: A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

SUBMITTER: Gilman AG

PROVIDER: S-EPMC283204 | biostudies-other | 1970 Sep

REPOSITORIES: biostudies-other

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A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Gilman A G AG

Proceedings of the National Academy of Sciences of the United States of America 19700901 1

A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP. ...[more]

PMID: 4318781

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