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Generation of reactive oxygen species by lethal attacks from competing microbes.

ABSTRACT: Whether antibiotics induce the production of reactive oxygen species (ROS) that contribute to cell death is an important yet controversial topic. Here, we report that lethal attacks from bacterial and viral species also result in ROS production in target cells. Using soxS as an ROS reporter, we found soxS was highly induced in Escherichia coli exposed to various forms of attacks mediated by the type VI secretion system (T6SS), P1vir phage, and polymyxin B. Using a fluorescence ROS probe, we found enhanced ROS levels correlate with induced soxS in E. coli expressing a toxic T6SS antibacterial effector and in E. coli treated with P1vir phage or polymyxin B. We conclude that both contact-dependent and contact-independent interactions with aggressive competing bacterial species and viruses can induce production of ROS in E. coli target cells.

SUBMITTER: Dong TG

PROVIDER: S-EPMC4343105 | biostudies-other | 2015 Feb

REPOSITORIES: biostudies-other

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Generation of reactive oxygen species by lethal attacks from competing microbes.

Dong Tao G TG Dong Shiqi S Catalano Christy C Moore Richard R Liang Xiaoye X Mekalanos John J JJ

Proceedings of the National Academy of Sciences of the United States of America 20150202 7

Whether antibiotics induce the production of reactive oxygen species (ROS) that contribute to cell death is an important yet controversial topic. Here, we report that lethal attacks from bacterial and viral species also result in ROS production in target cells. Using soxS as an ROS reporter, we found soxS was highly induced in Escherichia coli exposed to various forms of attacks mediated by the type VI secretion system (T6SS), P1vir phage, and polymyxin B. Using a fluorescence ROS probe, we foun ...[more]

PMID: 25646446

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