Unknown

Dataset Information

0

The nature of the autoimmune antibody repertoire in human immunodeficiency virus type 1 infection.


ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) seropositive donors typically have high serum antibody titers to a range of autoantigens, and the corresponding autoantibodies have been suggested to be of importance in the pathogenesis of HIV-1 infection. We have prepared 38 IgG human monoclonal autoantibodies from asymptomatic HIV-1 seropositive donors with elevated serum titers to autoantigens by construction of Fab combinatorial libraries on the surface of phage and affinity selection using a range of autoantigens, including double-stranded DNA, major histocompatibility complex class II, CD14, epidermal growth factor receptor, and ganglioside GD2. The autoantibodies are shown to be of moderate affinity and exhibit marked cross-reactivity with a range of antigens. This contrasts with the specific high-affinity antibodies selected (i) against infectious agents using the same libraries and (ii) against one of the autoantigens using a library from a donor with established autoimmune disease. The results lend no support to the presence of specific autoantibodies in HIV-1 infection and instead suggest attention should be focused on the pathological significance of high serum levels of antibodies capable of interacting with multiple molecular species.

SUBMITTER: Ditzel HJ 

PROVIDER: S-EPMC43651 | biostudies-other | 1994 Apr

REPOSITORIES: biostudies-other

altmetric image

Publications

The nature of the autoimmune antibody repertoire in human immunodeficiency virus type 1 infection.

Ditzel H J HJ   Barbas S M SM   Barbas C F CF   Burton D R DR  

Proceedings of the National Academy of Sciences of the United States of America 19940401 9


Human immunodeficiency virus type 1 (HIV-1) seropositive donors typically have high serum antibody titers to a range of autoantigens, and the corresponding autoantibodies have been suggested to be of importance in the pathogenesis of HIV-1 infection. We have prepared 38 IgG human monoclonal autoantibodies from asymptomatic HIV-1 seropositive donors with elevated serum titers to autoantigens by construction of Fab combinatorial libraries on the surface of phage and affinity selection using a rang  ...[more]

Similar Datasets

| S-EPMC1900112 | biostudies-literature
| S-EPMC7058406 | biostudies-literature
| S-EPMC2876609 | biostudies-literature
| S-EPMC136917 | biostudies-literature
| S-EPMC2789835 | biostudies-literature
| S-EPMC1310509 | biostudies-literature
| S-EPMC5760106 | biostudies-literature
| S-EPMC112575 | biostudies-literature
| S-EPMC6327975 | biostudies-literature
| S-EPMC3052598 | biostudies-literature