Ontology highlight
ABSTRACT:
SUBMITTER: Porchetta A
PROVIDER: S-EPMC4498449 | biostudies-other | 2015 Jul
REPOSITORIES: biostudies-other
Nano letters 20150610 7
Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH c ...[more]