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A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1?.

ABSTRACT: A novel dinuclear Ir(III) complex 5 was discovered to be specific to G-quadruplex DNA, and was utilized in a label-free G-quadruplex-based detection platform for transcription factor activity. The principle of this assay was demonstrated by using HIF-1? as a model protein. Moreover, this HIF-1? detection assay exhibited potential use for biological sample analysis.

SUBMITTER: Lu L

PROVIDER: S-EPMC4773817 | biostudies-other | 2016 Mar

REPOSITORIES: biostudies-other

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A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α.

Lu Lihua L Wang Modi M Mao Zhifeng Z Kang Tian-Shu TS Chen Xiu-Ping XP Lu Jin-Jian JJ Leung Chung-Hang CH Ma Dik-Lung DL

Scientific reports 20160302

A novel dinuclear Ir(III) complex 5 was discovered to be specific to G-quadruplex DNA, and was utilized in a label-free G-quadruplex-based detection platform for transcription factor activity. The principle of this assay was demonstrated by using HIF-1α as a model protein. Moreover, this HIF-1α detection assay exhibited potential use for biological sample analysis. ...[more]

PMID: 26932240

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