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Nutrikinetic study of genistein metabolites in ovariectomized mice.

ABSTRACT: This study was designed to evaluate the effect of ovariectomy on nutrikinetics of genistein metabolites. To characterize the time-dependent changes in genistein metabolite concentrations, we identified 13 genistein metabolites using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The nutrikinetics of the individual metabolites at different time points were analyzed. Nutrikinetic analysis showed that genistein, genistein 4'-glucuronide, genistein 7-glucuronide, 3-hydroxygenistein, and hippuric acid showed relatively high bioavailability in the sham group compared to that in the ovariectomy group, suggesting that ovariectomy likely results in lower genistein bioavailability. These results may be related to alteration of gut microbiota by ovariectomy. The relative abundance of species of the Parabacteroides, Dorea, and Butyricimonas genera, and Desulfovibrionaceae_unclassified, Lachnospiraceae_unclassified, and Rikenellaceae_unclassified families increased in the ovariectomy group while the relative abundance of 523_7_unclassified and Y52_unclassified_unclassified increased in the sham group. These results suggest that gut microbiota alteration by ovariectomy may affect genistein bioavailability.

SUBMITTER: Lee DH

PROVIDER: S-EPMC5653299 | biostudies-other | 2017

REPOSITORIES: biostudies-other

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Nutrikinetic study of genistein metabolites in ovariectomized mice.

Lee Da-Hye DH Kim Min Jung MJ Song Eun-Ji EJ Kim Jin Hee JH Ahn Jiyun J Nam Young-Do YD Jang Young-Jin YJ Ha Tae-Youl TY Jung Chang Hwa CH

PloS one 20171023 10

This study was designed to evaluate the effect of ovariectomy on nutrikinetics of genistein metabolites. To characterize the time-dependent changes in genistein metabolite concentrations, we identified 13 genistein metabolites using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The nutrikinetics of the individual metabolites at different time points were analyzed. Nutrikinetic analysis showed that genistein, genistein 4'-glucuronide, genistein 7-glucuronide ...[more]

PMID: 29059247

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Project description:Background:The prevalence of nonalcoholic steatohepatitis (NASH) in menopausal women is increasing, but current treatments have not been proven effective. The objective of this study was to investigate the treatment effects of genistein and running exercise in ovariectomized (OVX) rats with NASH.Methods:Thirty-six female Sprague-Dawley rats were divided into 6 groups, control; OVX with standard diet; OVX with high fat and high fructose (HFHF) diet for 4?weeks; OVX with HFHF and genistein treatment (16?mg/kg BW/day) for 5?weeks (OVX?+?HFHF+GEN); OVX with HFHF and moderate intensity exercise for 5?weeks (OVX?+?HFHF+EX); OVX with HFHF and combined treatments (OVX?+?HFHF+GEN?+?EX). Serum interleukin-6 (IL-6) levels, hepatic free fatty acid (FFA), hepatic glutathione (GSH), and hepatic malondialdehyde (MDA) levels were measured. Liver histology was examined to determine NASH severity.Results:OVX?+?HFHF group had the highest levels of hepatic FFA compared with OVX and control groups (5.92?±?0.84 vs. 0.37?±?0.01 vs. 0.42?±?0.04?nmol/mg protein, respectively, p?<?0.01). Serum IL-6 levels were significantly elevated in both OVX and OVX?+?HFHF groups as compared with controls (112.13?±?6.50 vs. 121.47?±?3.96 vs. 86.13?±?2.40?pg/mL, respectively, p?<?0.01). In OVX?+?HFHF group, hepatic MDA levels were higher, while GSH levels were lower than in OVX and control groups (MDA; 0.98?±?0.04 vs. 0.82?±?0.02 vs. 0.78?±?0.03?nmol/mg protein, and GSH; 46.01?±?0.91 vs. 55.21?±?1.40 vs. 57.94?±?0.32, respectively; p?<?0.01 for both). Comparing with OVX?+?HFHF group, rats that received genistein, exercise and combined treatments demonstrated an improvement in liver histopathology, decreased levels of hepatic FFA (1.44?±?0.21 vs. 0.45?±?0.04 vs. 0.49?±?0.05?nmol/mg protein, respectively, p?<?0.01), serum IL-6 (82.80?±?2.07 vs. 83.47?±?2.81 vs. 94.13?±?1.61?pg/mL, respectively, p?<?0.01), and hepatic MDA (0.80?±?0.03 vs. 0.76?±?0.02 vs. 0.76?±?0.03?nmol/mg protein, respectively, p?<?0.01).Conclusions:Genistein and moderate intensity exercise were effective in reducing the severity of NASH in OVX rats through the reduction in liver inflammation, oxidative stress and liver fat contents.

Dataset Information

Nutrikinetic study of genistein metabolites in ovariectomized mice.

Publications

Nutrikinetic study of genistein metabolites in ovariectomized mice.

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