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Triggering nucleic acid nanostructure assembly by conditional kissing interactions.

ABSTRACT: Nucleic acids are biomolecules of amazing versatility. Beyond their function for information storage they can be used for building nano-objects. We took advantage of loop-loop or kissing interactions between hairpin building blocks displaying complementary loops for driving the assembly of nucleic acid nano-architectures. It is of interest to make the interaction between elementary units dependent on an external trigger, thus allowing the control of the scaffold formation. To this end we exploited the binding properties of structure-switching aptamers (aptaswitch). Aptaswitches are stem-loop structured oligonucleotides that engage a kissing complex with an RNA hairpin in response to ligand-induced aptaswitch folding. We demonstrated the potential of this approach by conditionally assembling oligonucleotide nanorods in response to the addition of adenosine.

SUBMITTER: Azema L

PROVIDER: S-EPMC5814900 | biostudies-other | 2018 Feb

REPOSITORIES: biostudies-other

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Triggering nucleic acid nanostructure assembly by conditional kissing interactions.

Azéma Laurent L Bonnet-Salomon Servane S Endo Masayuki M Takeuchi Yosuke Y Durand Guillaume G Emura Tomoko T Hidaka Kumi K Dausse Eric E Sugiyama Hiroshi H Toulmé Jean-Jacques JJ

Nucleic acids research 20180201 3

Nucleic acids are biomolecules of amazing versatility. Beyond their function for information storage they can be used for building nano-objects. We took advantage of loop-loop or kissing interactions between hairpin building blocks displaying complementary loops for driving the assembly of nucleic acid nano-architectures. It is of interest to make the interaction between elementary units dependent on an external trigger, thus allowing the control of the scaffold formation. To this end we exploit ...[more]

PMID: 29272518

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