Ontology highlight
ABSTRACT:
SUBMITTER: Tian Y
PROVIDER: S-EPMC5921928 | biostudies-other | 2018 Apr
REPOSITORIES: biostudies-other
Chemical communications (Cambridge, England) 20180401 35
Three γ-heteroatom-substituted N-methylpyrroletetrazole-lysines (mPyTXKs) were synthesized and subsequently incorporated into proteins site-specifically via genetic code expansion. The γ-seleno-substituted derivative, mPyTSeK, showed excellent incorporation efficiency in Escherichia coli and allowed site-selective photo-cross-linking of the GST dimer. Furthermore, the mPyTSeK-cross-linked GST dimer can be cleaved under mild oxidative conditions. The incorporation of mPyTXKs into proteins in mamm ...[more]