Ontology highlight
ABSTRACT:
SUBMITTER: Dokshin GA
PROVIDER: S-EPMC6218216 | biostudies-other | 2018 Nov
REPOSITORIES: biostudies-other
Dokshin Gregoriy A GA Ghanta Krishna S KS Piscopo Katherine M KM Mello Craig C CC
Genetics 20180913 3
CRISPR-based genome editing using ribonucleoprotein complexes and synthetic single-stranded oligodeoxynucleotide (ssODN) donors can be highly effective. However, reproducibility can vary, and precise, targeted integration of longer constructs-such as green fluorescent protein tags remains challenging in many systems. Here, we describe a streamlined and optimized editing protocol for the nematode <i>Caenorhabditis elegans</i> We demonstrate its efficacy, flexibility, and cost-effectiveness by aff ...[more]