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717e6548-f49b-4907-b7a1-261a68164dae - samples

ABSTRACT: The Roche AlzheimerÃ¢Â€Â™s disease dataset (Roche_AD) consists of 80 samples from 40 unique individuals (one sample from the temporal cortex and one from deep white matter for each individual, 12 cases, 25 controls, 3 dementia). A total of 12,000 estimated cells from each sample were loaded on the 10x Single Cell Next GEM G Chip. cDNA libraries were prepared using the Chromium Single Cell 3Ã¢Â€Â™ Library and Gel Bead v3 kit according to the manufacturerÃ¢Â€Â™s instructions. cDNA libraries were sequenced using the Illumina NovaSeq 6000 System and NovaSeq 6000 S2 Reagent Kit v1.5 (100 cycles), aiming at a sequencing depth of minimum 30K reads/nucleus.

PROVIDER: EGAD00001009166 | EGA |

REPOSITORIES: EGA

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