Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies VII

ABSTRACT: Purpose: This study uses a high-throughput glycan microarray to evaluate the immunological evolution of antibodies to the glyco-antigen GD2. The goal is to determine germline and affinity mature antibody specificity and affinities/ Results: Affinity mature anti-GD2 antibodies 3F8 and ch14.18 had high affinity and were highly specific for the target GD2. Germline antibodies were also hihgly specific and had surprisingly high affinity. Conclusion: Antibodies to GD2 evolved from highly specific germlines. Highly specific germlines may be critical in evading autoimmunity issues.

ORGANISM(S): Dermestes lardarius Bos taurus Mus musculus Sus scrofa domesticus Homo sapiens Human immunodeficiency virus 1 Oryctolagus cuniculus Trypanosoma cruzi Frangula alnus Gossypium hirsutum Pachymenia carnosa Quercus alba Ceratonia siliqua Rhodotorula Ovis aries Pandalus borealis Megathura crenulata Senegalia senegal Gallus gallus Bothrops moojeni Oncorhynchus nerka

PROVIDER: GSE100310 | GEO | 2017/08/25

SECONDARY ACCESSION(S): PRJNA391739

REPOSITORIES: GEO