Project description:Elucidation of the wound response genes in Drosophila embryos Keywords: response to wounding wild-type drosophila embryos were wounded by laser ablation and compared to unwounded embryos.
Project description:Transcriptional profiling of laser wounded syncytial Drosophila embryos compared with control unwounded embryos. Early Drosophila embryos (Nuclear cycle 4-6) were collected, either laser wounded or not wounded, and then collected and processed immediately. Wounded and unwounded embryos express sGMCA (actin reporter)
Project description:Transcriptional profiling of laser wounded syncytial Drosophila embryos compared with control unwounded embryos. Early Drosophila embryos (Nuclear cycle 4-6) were either laser wounded or not wounded, and then collected and processed after 30 minutes. Wounded and unwounded embryos express sGMCA (actin reporter).
Project description:Drosophila embryos that were lacking the hemocyte lineage were compared to wild-type embryos. Keywords: genetic modification Wild-type drosophila embryos were compared to serpent mutant embryos.
Project description:This SuperSeries is composed of the following subset Series: GSE10207: wounded versus nonwounded drosophila embryos GSE10208: hemocyte minus versus hemocyte plus drosophila embryos GSE10209: wounded vs unwounded hemocyte deficient embryos Keywords: SuperSeries Refer to individual Series
Project description:Combination of both sterile wounding and infection may lead to severe health defects, revealing the importance of the balance between the intensity and resolution of the inflammatory response for the organisms fitness. Underlying mechanisms remain however elusive. Using Drosophila, we analyzed the very first steps of the process by comparing the transcriptome landscape of infected (simple hit flies, SH), wounded and infected (double hit flies, DH) and wounded (control) flies. Our objective was to identify the molecular mechanisms underlying the susceptibility of wounded flies to combined trauma and bacterial infection.
Project description:To identify Aub function in mRNA regulation in the Drosophila embryo, we have performed mass spectrometry analysis of Aub interactors, following immunoprecipitation of GFP-Aub in 0-2 hour-embryos. Immunoprecipitation of GFP alone was used as negative control. Because Aub accumulates at high levels in the germ plasm, GFP-Aub immunoprecipitation was also performed in oskar mutant embryos that do not assemble the germ plasm. Proteins coprecipitating with GFP-Aub were similar in wild-type and oskar mutant embryos. Translation factors were enriched among proteins coprecipitating with Aub.