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Unknown,Transcriptomics,Genomics,Proteomics

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Expression study of 'hemocyte minus vs hemocyte plus, and wounded vs unwounded hemocyte deficient' drosophila embryos

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE10207: wounded versus nonwounded drosophila embryos GSE10208: hemocyte minus versus hemocyte plus drosophila embryos GSE10209: wounded vs unwounded hemocyte deficient embryos Keywords: SuperSeries Refer to individual Series

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Mark Winfield

PROVIDER: E-GEOD-10225 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos.

Stramer Brian B Winfield Mark M Shaw Tanya T Millard Thomas H TH Woolner Sarah S Martin Paul P

EMBO reports 20080314 5

By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated ...[more]

PMID: 18344972

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hemocyte minus versus hemocyte plus drosophila embryos

Project description:Drosophila embryos that were lacking the hemocyte lineage were compared to wild-type embryos. Keywords: genetic modification Wild-type drosophila embryos were compared to serpent mutant embryos.

2010-05-22 | E-GEOD-10208 | biostudies-arrayexpress

wounded vs unwounded hemocyte deficient embryos

Project description:Wounded versus unwounded embryos lacking the hemocyte lineage were compared. Keywords: wounded vs unwounded srp mutant embryos Serpent mutant embryos were wounded by laser ablation and compared to unwounded.

2010-05-22 | E-GEOD-10209 | biostudies-arrayexpress

Telomerase inhibitor TMPyP4 induced gene expression

Project description:The effect of TMPyP4, the well established anticancer telomerase inhibitor on genome wide expression of genes was investigated Keywords: Time course Cells were treated with TMPyP4 for either 24 or 48 hours before RNA isolation.Untreated cells were taken as controls and the TMPyP4 treated cells as treatments. Experiments at each time point were replicated four times.

2010-06-10 | E-GEOD-5226 | biostudies-arrayexpress

Telomerase inhibitor TMPyP4 induced widespread gene expression

Project description:The effect of TMPyP4, the well established anticancer telomerase inhibitor on genome wide expression of genes, analyzed across different cell-lines, was investigated Keywords: Comparative gene-expression profiling Cells were treated with TMPyP4 for 48 hours before RNA isolation. Untreated cells were taken as controls and the TMPyP4 treated cells as treatments. Experiments in each cell-line (A549 and HeLa S3) were replicated four times.

2010-06-20 | E-GEOD-9710 | biostudies-arrayexpress

Identification of EMT related genes in mcf10 cell line

Project description:In vitro studies on immortalized breast MCF10A cells were additionally performed to assess the phenotypic and genetic changes associated to mesenchymal transformation of breast cells and to their epithelial plasticity. To further study the molecular events associated to the cell plasticity of MCF10A cells, we decided to characterize the genetic programs modulated during the phenotypic changes by cDNA microarrays analysis. Cells were grown to 10-20% (sparse) or 80-100% confluence (confluent), and the corresponding RNAs were extracted and hybridized onto cDNA microarrays.

2010-05-25 | E-GEOD-8430 | biostudies-arrayexpress

Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte and fat body transcriptomes

Project description:This SuperSeries is composed of the following subset Series: GSE16775: Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte transcriptome GSE16776: Effect of HdIV or MdBV injection on the Spodoptera frugiperda fat body transcriptome Refer to individual Series

2011-04-05 | E-GEOD-17293 | biostudies-arrayexpress

wounded versus nonwounded drosophila embryos

Project description:Elucidation of the wound response genes in Drosophila embryos Keywords: response to wounding wild-type drosophila embryos were wounded by laser ablation and compared to unwounded embryos.

2008-04-01 | E-GEOD-10207 | biostudies-arrayexpress

Involvement of JunD in the antiproliferative effect of 9-tetrahydrocannabinol on human breast cancer cells 1

Project description:THC induces an activation of JunD, by upregulation and translocation to the nuclear compartment, which is accompanied by a decrease in cell proliferation Keywords: THC, JUND Total RNA was isolated from cells treated with vehicle (DMSO), 3 or 5 ïM THC for 8 or 24h, and non-treated cells at the time of drug addition (t=0, reference) using RNeasy Mini Kit (Qiagen, Hilden, Germany). Double strand cDNA was amplify with Superscript Choice System and T7-(deoxythymidine)24 oligo-primers (Life Technologies, Inc., Gaithersburg, MD) and in vitro transcription was carried out with MegascriptTM T7 (Ambion, Austin, TX). For each condition, half of the replicates were labeled with Cy5-dUTP fluorochrome (Amersham, Uppsala, Sweden) and the reference with Cy3-dUTP fluorochrome (Amersham), and the other half were dye swapped. Hybridization was performed on a new version of the Spanish National Cancer Research Center (CNIO) Oncochip manufactured by the CNIO Genomic Unit (http://bioinfo.cnio.es/data/oncochip), which contains 9.726 clones corresponding to 6,386 different genes. Slides were scanned in a Scanarray 5000 XL scanner (GSI Lumonics Kanata, Ontario, Canada) and quantified using the GenePix Pro 6.0 program (Axon Instruments Inc., Union City, CA).

2010-10-08 | E-GEOD-8502 | biostudies-arrayexpress

E-cadherin and EMT regulation by E47 transcription represor

Project description:Expression profile analysis in MDCK-E47 cells in comparisom with MDCK CMV control cells Keywords: Genetic Modification (overexpression of E-cadherin repressors) The Oncochip microarray platform v1.1 contains 9,726 clones corresponding to 6,386 different genes, and it includes 2,489 duplicate clones. Duplicate samples (different extrations of RNA) were labeled with dUTP-Cy5 and hybridized against dUTP-Cy3-MDCK control cells. In addittion, one RNA extraction was labelled with dUTP-Cy3 and was hybridized againts dUTP-Cy5- CMV control cells

2010-06-24 | E-GEOD-9147 | biostudies-arrayexpress

Functional characterization of E- and P-cadherin in invasive breast cancer cells

Project description:Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood. Methods: To compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation. Expression of either E- and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E- and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E- and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type. Microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins using different clones for each conditions to reveal that these molecules can activate signaling pathways leading to significant changes in gene expression

2010-06-19 | E-GEOD-13362 | biostudies-arrayexpress

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