Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Adult functions for the Drosophila DHR78 nuclear receptor

ABSTRACT: Here we study the adult functions of the single Drosophila member of this subfamily, DHR78, with the goal of defining its ancestral functions in the absence of genetic redundancy. We show that DHR78 mutants have a shortened lifespan and reduced motility. Mated DHR78 mutant females display reduced triglycerides along with a reduced feeding rate. Transcriptional profiling reveals a major role for DHR78 in promoting the expression of genes that are abundantly expressed in the midgut, suggesting that it contributes to nutrient uptake. We also identify roles for DHR78 in maintaining the expression of genes in the ecdysone and Notch signaling pathways.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE106866 | GEO | 2018/02/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

EcR mutant midgut comparison

Project description:Comparisons between EcR mutant midgut and a reference control sample from mixed-stage normal midgut Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

2003-07-23 | GSE540 | GEO

EcR mutant midgut comparison

2010-06-05 | E-GEOD-540 | biostudies-arrayexpress

Midgut metamorphosis timecourse

Project description:A midgut reference sample is compared to midgut samples taken at various stages before (18h, 4h), at (0h), and after (2h, 3h, 4h, 5h, 6h, 8h, 10h, 12h) puparium formation. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

2003-07-23 | GSE543 | GEO

Whole animal vs. tissues at 18h before puparium formation

Project description:Comparisons between whole animal and epidermis with attached muscle, salivary gland, wing disc, midgut, and central nervous system tissues at approximately 18 hours before pupariation. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

2003-07-23 | GSE541 | GEO

18h before puparium formation vs. puparium formation tissue comparisons

Project description:Samples at 18h before puparium formation were compared to samples at puparium formation for each the following tissues: epidermis with attached muscle, salivary gland, wing disc, midgut, and central nervous system. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development

2010-06-05 | E-GEOD-542 | biostudies-arrayexpress

18h before puparium formation vs. puparium formation tissue comparisons

2003-07-23 | GSE542 | GEO

sage gene RNAi experiment

Project description:Comparisons between the salivary glands upon the RNAi of sage gene vs. the control. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development

2010-06-05 | E-GEOD-283 | biostudies-arrayexpress

sage gene RNAi experiment

2003-07-23 | GSE283 | GEO

Drosophila E78A nuclear receptor regulates intestinal dietary triacylglyceride uptake and systemic lipid levels

Project description:Here we show that the Drosophila nuclear receptor E78A regulates transcription in adult animals facilitating dietary lipid uptake. E78A mutant adults are viable with normal developmental timing, locomotor activity, female fecundity, but have significantly reduced stored triglycerides. This reduction in whole body triglyceride stores appears to originate from the midgut, using neutral lipid stainings we determined lipid levels in the midgut were greatly reduced. Upon examining our RNA-seq analysis we found that a gastric lipase, CG17192, is transcriptionally coordinated by E78A. CG17192 is expressed in an adult, intestinal specific fashion within control flies, and has a human ortholog. Through dietary supplementation and genetic testing we determined that the whole body hypolipidemia seen within E78A mutants is dependent on CG17192 expression within the intestine. Restoration of this lipase in the intestine of mutant flies is sufficient to restore normal lipid levels. Our data support the model that E78A contributes to a transcriptional regulation of the gastric lipase, CG17192, thus directing dietary triglyceride uptake in the adult fly.

2021-01-01 | GSE152031 | GEO