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Whole genome mapping of DNA G-quadruplexes in multiple species by G4-seq

ABSTRACT: The identification of DNA G-quadruplexes (G4s) in the genome is important to study different biological processes in which these structures play a role, such as genome rearrangement, transcriptional regulation and DNA replication. G4-seq allowed the high-throughput experimental mapping of G-quadruplexes in the human genome. We developed here an improved version of this method, named G4-seq2, which we applied to generate G-quadruplexes genomic maps for 12 species, selected as important models organism to study development or as pathogens of clinical relevance. Those multi-species maps, publicly available for the community, will allow to further understand the design principle of G-quadruplex formation in genomic context, to study G-quadruplex biology in those model organisms, to predict ligand targeting for therapeutic usage and to design G-quadruplex computational predictors based on genome-wide experimental measurements.

ORGANISM(S): Trypanosoma brucei Escherichia coli Arabidopsis thaliana Danio rerio Leishmania major Mus musculus Homo sapiens Saccharomyces cerevisiae Plasmodium falciparum Caenorhabditis elegans Cereibacter sphaeroides Drosophila melanogaster

PROVIDER: GSE110582 | GEO | 2018/08/01

REPOSITORIES: GEO

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