Dataset Information

Newly identified interferon tau-responded cytidine/uridine monophosphate kinase 2 and Hes family BHLH transcription factor 4 genes in bovine peripheral blood cells at peri-implantation period

ABSTRACT: In cattle, interferon-stimulating genes (ISGs) such as ISG15, MX1, MX2, and OAS1 are known as classical ISGs that are intensely involved in the implantation process. Various molecules play a crucial role in the mechanism underlying ISG effects. Although microarray analysis highlighted the expression of various molecules during the implantation period, these are incompletely characterized. In the present study, some specifically expressed genes were selected and their characteristics were examined. The microarray data of peripheral blood leucocytes from artificially inseminated and embryo-transferred cows were used to identify new ISG candidates. Seven common genes, including ISG15 and OAS1, were confirmed, but only four of these were amplified by reverse transcription quantitative polymerase chain reaction. These showed significantly higher expression in pregnant cows than in non-pregnant cows. Moreover, three expressed sequence tags (ESTs) showed significantly higher expression in granulocytes after IFNT stimulation. NCBI BLAST analysis revealed the similar sequences of two ESTs on chromosome 16 that were deemed as Hes family BHLH transcription factor 4 (HES4). Another EST was identified on chromosome 11 as cytidine/uridine monophosphate kinase 2 (CMPK2). The promoter sequences of HES4 and CMPK2 were cloned and analyzed from DNA of whole blood cells in cyclic Holstein cows. In silico analysis facilitated the identification of the transcription factor-binding sequences, including interferon-stimulated response element- and interferon regulatory factor-binding sites, on the promoter region of HES4 and CMPK2. These genes may be one of the new ISGs related to embryo implantation and may participate in the coordination of feto-maternal interface in cows.

ORGANISM(S): Bos taurus

PROVIDER: GSE112490 | GEO | 2018/03/30

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:HIV is able to outpace the innate immune response, including the response mediated by interferon (IFN), to establish a productive infection. However, monocyte derived macrophages (MDMs) may be protected from HIV infection by treatment with type I IFN before virus exposure. The ability of HIV to modulate the type I IFN-mediated innate immune response when it encounters a cell that has already been exposed to IFN was investigated. To investigate the presence of HIV on an established IFN response, MDMs were subjected to four different conditions: (1) IFN-treated only, (2) IFN-treated followed by HIV infection, (3) HIV infected only, and (4) a mock-treated and mock-infected control. Microarray gene expression analysis was performed on a total of 24 samples derived from the 4 conditions assessed at 3 time points (1, 4 and 8 days following treatment/infection) for both IFN-α2 or -ω. Initially, ISGs were identified as those that were upregulated greater than 2-fold by IFN alone (condition 1) at both Days 4 and 8. Then, the IFN-treated condition was compared to the IFN-treated followed by HIV-infection condition in order to identify those ISGs that were downregulated at least 1.5-fold by the presence of HIV at both days. Assuming that it would be counterproductive for HIV infection by itself to induce the expression of ISGs with putative anti-HIV effects, those ISGs that were upregulated greater than 2-fold in the HIV control were removed. Finally, ISGs that passed these filters and were concordant with both IFN-treatments (IFN-α2 and -ω) were identified and corresponded to the following 8 ISGs: AXL receptor tyrosine kinase (AXL), interferon-alpha inducible protein 27 (IFI27), interferon-induced protein 44 (IFI44), interferon-induced protein 44-like (IFI44L), ISG15, OAS1, OAS3 and XIAP associated factor 1 (XAF1). It should be noted that the IFN-α2 and -ω microarray experiments were performed in different batches but batch effects were not corrected since genes were identified by the filtering approach just described within each batch.

Project description:Background: In food production animals, especially cattle, the diagnosis of gestation is an important issue because the timing of gestation has direct effects on the running of farms. Various methods have been used to detect gestation, but none of them are ideal because of problems with the timing of detection, or the accuracy, simplicity, or cost of the method, etc. A new method for detecting gestation, which involves assessing interferon-tau-related gene expression in peripheral blood leucocytes (PBL), was recently proposed. PBL fractionation methods were used to examine whether the expression profiles of various PBL populations could be used as reliable diagnostic markers of bovine gestation. Methods: PBL were collected on days 0 (just before artificial insemination), 7, 14, 17, 21, and 28 of gestation. The gene expression levels of the PBL were assessed with microarray analysis and/or quantitative real-time reverse transcription (q)-PCR. PBL fractions were collected with flow cytometry or density gradient cell separation using Histopaque 1083 or Ficoll-Conray solution. The expression levels of four interferon-tau-related genes, interferon-stimulated protein 15 kDa (ISG15), myxovirus-resistance (Mx) 1 and 2, and 2'-5'-oligoadenylate synthetase (OAS1), were then analysed in each fraction until day 28 of gestation using qPCR. Results: Miroarray analysis detected 72 and 28 genes significantly higher in whole PBL on days 14 and 21 of gestation compare to those on day 0, respectively. They involved interferon-tau responded genes. The expression levels of these genes increased with the progression of gestation. In the flow cytometry experiment, on day 14 all of the genes displayed significantly higher expression levels in the granulocyte fraction than the other fractions, and their expression levels gradually decreased until day 28 of gestation. Strong correlations were observed between the expression levels of the four genes in the granulocyte fractions obtained with flow cytometry and density gradient separation. Conclusions: The expression profile of the four abovementioned genes could be a useful diagnostic biomarker of bovine gestation. Assessing the expression levels of ISG15, Mx1, Mx2 and OAS1 in a granulocyte fraction obtained with density gradient separation is a practical way of detecting gestation in cows within three weeks of insemination. Gene expression profiles were analyzed in the peripheral blood leucocytes at days 0 (n=5), 14 (n=3) and 21 (n=5) after artificial insemination in cow.

Dataset Information

Newly identified interferon tau-responded cytidine/uridine monophosphate kinase 2 and Hes family BHLH transcription factor 4 genes in bovine peripheral blood cells at peri-implantation period

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets