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Nucleosome positioning - Transient Depletion and Recovery of the Essential RSC Chromatin Remodeling Complex

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE118214 | GEO | 2018/12/31

REPOSITORIES: GEO

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Nucleosome positioning - Transient Depletion and Recovery of the Essential RSC Chromatin Remodeling Complex [RNA-Seq]

Project description:we use an induced degron system to study how rapid clearance of ATP-dependent chromatin remodelers affect nucleosome locations. 1. Screening eight remodelers 2. depletion of Sth1 leads to rapid and reversible fill-in of nucleosome-free regions at gene promoters. 3. changes at depletion and recovery do not depend on major chromatin reassembly during DNA replication. 4. RSC is crucial in maintaining open promoters of low expressed genes. RSC is not directly required for an acute transcriptional response. 5. Changes in nucleosome positions are tightly related to the specific TSS usage at promoters.

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Nucleosome positioning - Transient Depletion and Recovery of the Essential RSC Chromatin Remodeling Complex [MNase-Seq]

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Nucleosome positioning - Transient Depletion and Recovery of the Essential RSC Chromatin Remodeling Complex

Project description:Nucleosome positioning - Transient Depletion and Recovery of the Essential RSC Chromatin Remodeling Complex

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Dynamics of Chromatin and Transcription during Transient Depletion of the RSC Chromatin Remodeling Complex.

Project description:Nucleosome organization has a key role in transcriptional regulation, yet the precise mechanisms establishing nucleosome locations and their effect on transcription are unclear. Here, we use an induced degradation system to screen all yeast ATP-dependent chromatin remodelers. We characterize how rapid clearance of the remodeler affects nucleosome locations. Specifically, depletion of Sth1, the catalytic subunit of the RSC (remodel the structure of chromatin) complex, leads to rapid fill-in of nucleosome-free regions at gene promoters. These changes are reversible upon reintroduction of Sth1 and do not depend on DNA replication. RSC-dependent nucleosome positioning is pivotal in maintaining promoters of lowly expressed genes free from nucleosomes. In contrast, we observe that upon acute stress, the RSC is not necessary for the transcriptional response. Moreover, RSC-dependent nucleosome positions are tightly related to usage of specific transcription start sites. Our results suggest organizational principles that determine nucleosome positions with and without RSC and how these interact with the transcriptional process.

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Project description:Nucleosome positioning - Transient Depletion and Recovery of the Essential RSC Chromatin Remodeling Complex [RNA-Seq]

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