Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The in vitro influence of orexin B on the porcine myometrium on days 15 to 16 of gestation.

ABSTRACT: Orexin A (OXA) and orexin B (OXB) are hypothalamic-derived peptides that participate in the regulation of energy metabolism, food intake and reproductive function by influencing the hypothalamic-pituitary-ovarian axis. Orexins are also produced in the endometrium, myometrium and placenta, which suggests that they could act as a link between energy metabolism and the reproductive system. The aim of this study was to explore the influence of orexin B on global gene expression in the porcine myometrium during early gestation, on days 15 to 16 of pregnancy (implantation period).

ORGANISM(S): Sus scrofa Sus scrofa domesticus

PROVIDER: GSE122414 | GEO | 2018/11/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The in vitro influence of orexin A on the porcine endometrium on days 15 to 16 of gestation.

Project description:Orexin A belongs to the group of hypothalamic-derived peptides that are involved in a number of processes, such as regulation of energy metabolism, control of food intake and regulation of the reproductive system. Orexin A is also expressed in the endometrium, myometrium and placenta, which suggests that it may act as an important local regulator of the reproductive functions. The aim of this study was to explore the influence of orexin A on global gene expression in the porcine endometrium during early gestation, on days 15 to 16 of pregnancy (implantation period).

2018-06-02 | GSE115195 | GEO

The in vitro effect of orexin A on the porcine myometrial transcriptomic profile during early-implantation period

Project description:Orexins belong to the group of metabolism regulating factors connected mainly with the nervous system. Both biologically active neuropeptides, orexin A (OXA) and orexin B (OXB) are products of the proteolytic cleavage of common 130 amino-acid precursor – prepro-orexin (PPO). In the reproductive system orexins has been found to act not only on the endocrine, but also auto- and paracrine manner. In the present study we focused on the effect of OXA treatment on the global gene expression in the porcine myometrium during the most dynamic phase of the early pregnancy – the implantation period (days 15 to 16 of gestation).

2018-11-09 | GSE122310 | GEO

The in vitro influence of orexin B on the porcine endometrium on days 15 to 16 of gestation.

Project description:Orexins, namely orexin A (OXA) and orexin B (OXB) belongs to the family of neuropeptides produced mainly by specific neurons of the lateral region of the hypothalamus. Primarily orexins were recognized as hormones involved exclusively in the regulation of food intake and energy homeostasis. However, a growing body of evidences indicate their involvement in the regulation of an increasing number of biological processes, inter alia, in the regulation of the reproductive functions. The aim of this study was to explore the influence of orexin B on global gene expression in the porcine endometrium during early gestation, on days 15 to 16 of pregnancy (implantation period).

2018-11-14 | GSE122415 | GEO

Gene profiling within the orexin-producing neurons

Project description:Narcolepsy is a sleep disorder characterized by excessive daytime sleepiness and attacks of muscle atonia triggered by strong emotions (cataplexy). The best biological marker of narcolepsy is orexin deficiency with dramatic loss in hypothalamic orexin-producing neurons. Together with a tight HLA and T-cell receptor alpha(5) association, narcolepsy is believed to be autoimmune although all attempts to prove it have failed.To characterize orexin specific peptides we produced a transgenic mouse model to access to the orexin neurons transcription profile. We generated BAC-based transgenic mice by replacing the orexin coding sequence by a flag-tagged poly(A) binding protein (Pabp1) cDNA sequence. The basis of this construct is to take advantage of the ability of Pabp1 to bind to the poly(A) tails of mRNAs in vivo. Thus mRNAs from orexin cells are expected to be enriched by cross-linking them to the flag-tagged PABP and then co-immunoprecipitating this complex with a specific anti-flag monoclonal antibody. Keywords: cell type comparison

2010-02-15 | GSE17617 | GEO

Gene profiling within the orexin-producing neurons

2010-02-24 | E-GEOD-17617 | biostudies-arrayexpress

The in vitro influence of orexin B on the porcine myometrium on days 15 to 16 of gestation.

Project description:The in vitro influence of orexin B on the porcine myometrium on days 15 to 16 of gestation.

| PRJNA505072 | ENA

Orexin/Hypocretin Receptor 2 Signaling in MCH Neurons Regulates REM sleep and Insulin Sensitivity

Project description:Orexin/hypocretin receptor type 2 (Ox2R), which is widely expressed in the brain, receives orexin signals and modulates sleep and metabolism. Ox2R selective agonists are currently under clinical trials for narcolepsy treatment. Nevertheless, it remains unclear whether Ox2R exerts an inhibitory effect via Gi proteins in addition to an excitatory effect via Gq proteins. Here, we focused on Ox2R expression and function in MCH neurons, which have opposite roles to orexin neurons in sleep and metabolism regulation. Ox2R-expressing MCH neurons showed heterogeneity of RNA expression, and orexin B application in brain slices induced both excitatory and inhibitory responses in distinct MCH neuron populations. Ox2R inactivation in MCH neurons reduced transitions from NREM to REM sleep and impaired insulin sensitivity with hyperphagia. In conclusion, Ox2R mediates excitatory and inhibitory responses in MCH neuron subpopulations in vivo, which might regulate sleep and metabolism.

2024-11-19 | GSE281229 | GEO

Human chorionic gonadotropin modulates the transcriptome of the myometrium and cervix in late gestation

Project description:Murine myometrium and cervix samples were profiled using paired-end Illumina RNA-seq to evaluate transcriptomic changes in these tissues associated with the systemic administration of hCG.

2021-03-05 | GSE157795 | GEO

Identification of the hypothalamic neuronal populations that derive from Pomc-expressing precursors

Project description:Hypothalamic neuronal populations are central regulators of energy homeostasis and reproductive function. However, the ontogeny of these critical hypothalamic neuronal populations is largely unknown. Here, we reveal novel cellular fates of the hypothalamic Pomc-expressing precursors by combining mouse genetics with a conditional viral ribosome-tagging approach to phenotype neurons. Our results show that the Pomc-expressing precursors differentiate into discrete neuronal subpopulations that mediate not only energy balance (POMC and AgRP) but also reproductive physiology (Kisspeptin).

2015-04-16 | GSE56917 | GEO

Global Analysis of Gene Expression Mediated By Orexin Receptor 1 Signaling in a Hypothalamic Cell Line

Project description:The orexins/hypocretins and their cognate G-protein coupled receptors have been widely studied due to their associations with various behaviors and cellular processes. However, the detailed downstream signaling cascades that mediate these effects are not completely understood. We report the generation of a neuronal model cell line that stably expresses orexin receptor 1 and an RNA-Seq analysis of changes in gene expression seen upon receptor activation. Upon treatment with orexin, several related families of related transcription factors are transcriptionally regulated, including the early growth response genes (Egr), the Kruppel-like factors (Klf), and the Nr4a subgroup of nuclear hormone receptors. Furthermore, the transcriptional effects observed showed a degree of similarity with data from in vivo sleep deprivation microarray studies, supporting the physiological relevance of the data set. These results provide new insight into the molecular signaling events that occur during orexin receptor 1 signaling and support a role for orexin signaling in the stimulation of wakefulness during sleep deprivation studies.

2018-02-13 | GSE99690 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data