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In utero lead (Pb) exposure and neuron-specific DNA methylation in mice

ABSTRACT: Lead (Pb) exposure is associated with a wide range of neurological deficits. Epigenetic changes, such as DNA methylation, may be impacted by environmental exposures and can affect neurodevelopmental outcomes over the life-course. Mating mice were obtained from a C57BL/6J background agouti Avy strain. Virgin dams (a/a) were randomly assigned exposure to 2.1 ppm (low) or 32 ppm (high) Pb-acetate through the drinking water, started two weeks prior to mating with viable yellow agouti male mice (Avy/a) and continued throughout gestation and three weeks after birth. At 10 months of age, we separated brain NeuN+ (a marker for neuronal nuclei) neuronal nuclei from NeuN non-neuronal nuclei in mice. We investigated neuron specific genome-wide promoter DNA methylation associated with early-life Pb exposures using the Roche NimbleGen Mouse DNA methylation 3x720K CpG island Promoter Array.

ORGANISM(S): Mus musculus

PROVIDER: GSE125174 | GEO | 2019/04/12

REPOSITORIES: GEO

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