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Genome-wide maps of chromatin state in Ad-ube3b and control lymphoma cells

ABSTRACT: We report the application of single-molecule-based sequencing technology for high-throughput profiling of p-RNAPII(ser2) with promoters in mammalian cells. We performed ChIP sequencing with antibody to total p-RNAPII(ser2) in UBE3B overexpression and control group. Inspection of multiple individual traces and global analyses showed that UBE3B overexpression globally reduced association of p-RNAPII(ser2) with promoters.

ORGANISM(S): Homo sapiens

PROVIDER: GSE126746 | GEO | 2020/10/02

REPOSITORIES: GEO

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Genome-wide maps of chromatin state in Ad-UBE3B and control lymphoma cells

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of RNAPII with promoters in mammalian cells. We performed ChIP sequencing with antibody to total RNAPII in UBE3B overexpression and control group. Inspection of multiple individual traces and global analyses showed that UBE3B overexpression globally reduced association of RNAPII with promoters.

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Genome-wide maps of chromatin state in TRIB3Cas9 and control lymphoma cells

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Next Generation Sequencing Facilitates Quantitative Analysis of ad-control and ad-ube3b cells' Transcriptomes in lymphoma

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