Project description:Analysis of effect of hindlimb suspension and reloading on C57Bl/6 mouse soleus muscle. Experimental groups examined: -Control mice, 14 days -Hindlimb suspension for 7 days -Hindlimb suspension for 7 days, and subsequent reloading for 1 day -Hindlimb suspension for 7 days, and subsequent reloading for 7 days Keywords: other
Project description:Analysis of effect of hindlimb suspension and reloading on C57Bl/6 mouse soleus muscle. Experimental groups examined: -Control mice, 14 days -Hindlimb suspension for 7 days -Hindlimb suspension for 7 days, and subsequent reloading for 1 day -Hindlimb suspension for 7 days, and subsequent reloading for 7 days
Project description:1 year-old male 129/SV mice. Animals were acclimatized to housing in single cages for one week followed by 7 days of hindlimb suspension and one day of reloading. Soleus muscle was harvested, total RNA isolated and subjected to transcript profiling with custom microarrays with custom-designed ATLASTM cDNA nylon filter as described (Dapp, C., Schmutz, S., Hoppeler, H. & Fluck, M. (2004) Physiol Genomics 20, 97-107). Keywords: transcriptome
Project description:1 year-old male 129/SV mice. Animals were acclimatized to housing in single cages for one week followed by 7 days of hindlimb suspension and one day of reloading. Soleus muscle was harvested, total RNA isolated and subjected to transcript profiling with custom microarrays with custom-designed ATLASTM cDNA nylon filter as described (Dapp, C., Schmutz, S., Hoppeler, H. & Fluck, M. (2004) Physiol Genomics 20, 97-107). Keywords: transcriptome Six biological replicas
Project description:To investigate the molecular mechanisms governing the transition of skeletal muscle from atrophy to compensatory regeneration and hypertrophy, we employed a mouse model involving hindlimb unloading and subsequent reloading, conducting a comprehensive analysis of global gene expression using RNA-sequencing (RNA-seq). Gastrocnemius muscle samples were obtained from three groups: control mice, mice subjected to 10 days of hindlimb unloading-induced muscle atrophy, and mice reintroduced to normal cage activity for 1 day following the unloading period (reloading).
Project description:Examination of effect of mechanical loading induced by hindlimb suspension, and subsequent reloading, in soleus muscle in 14 day old female pathogen–free Wistar rats. Keywords = skeletal muscle Keywords = atrophy Keywords: other
Project description:Examination of effect of mechanical loading induced by hindlimb suspension, and subsequent reloading, in soleus muscle in 14 day old female pathogenâfree Wistar rats. Keywords = skeletal muscle Keywords = atrophy