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Macrophages + Vitamin A

ABSTRACT: We are interested in whether peritoneal LCM are programmed by omental Wt1+ stroma (identified as PDPN+ cells). To this end, we co-cultured omental PDPN+ cells (purified by MACS by depleting CD45+ and CD31+ cells) with peritoneal exudates in a manner that enables cell:cell contact or inhibited this with a transwell insert or enabled cell:cell contact in the presence of the retinoic acid receptor inverse agonist BMS493. Peritoneal exudates were also cultured alone or with retinoic acid (1uM). After 5-7 days PDPN+ cells and F480+ cells were FACS sorted from cultures directly into RLT Buffer. Ex vivo peritoneal LCM were also FACS-sorted for comparison. PRJ0019587

ORGANISM(S): Mus musculus

PROVIDER: GSE130815 | GEO | 2019/07/16

REPOSITORIES: GEO

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