Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CHO_library_B_GCN Screen

ABSTRACT: CRISPR screen targeting 3022 CHO (Chinese hamster) genes to identify those contributing to induction of the Integrated Stress Response by histidinol treatment.

ORGANISM(S): Cricetulus griseus synthetic construct

PROVIDER: GSE134917 | GEO | 2019/07/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

A Chinese hamster transcription start site atlas that enables targeted editing of CHO cells

Project description:Gene expression is a key determinant of phenotypes that made Chinese Hamster Ovary (CHO) cells, with their human-like glycosylation profile and high protein titers, one of the most widely used cells for the production of therapeutic proteins and biopharmaceuticals. Engineering CHO gene expression thus holds a key to improve drug quality and cost effective production. However, the success of engineering gene expression or ectopic activation of silent genes to optimize desired pathways requires accurate annotation of the underlying regulatory elements and the transcription start site (TSS). Unfortunately, to date, most TSSs of CHO-expressed genes and the ~50% of hamster genes that are silent in CHO were computationally predicted and are frequently inaccurate. To oust this hurdle, we report revised TSSs annotations for 15,308 Chinese Hamster genes and 4,145 non-coding RNAs based on experimental data from CHO K1 cells and 10 hamster tissues. In the example of the glycosyltransferase gene Mgat3, we further demonstrate how accurate annotations readily facilitate activating silent genes by CRISPRa. Together, we envision that our annotation and data from the Chinese Hamster will provide a rich resource for the CHO community, improve genome engineering efforts and additionally aid comparative and evolutionary studies.

2020-10-06 | GSE159044 | GEO

Comparison of transcriptional profile of CHO cells cultured in different media.

Project description:Two clones of Chinese hamster ovary (CHO) cells were cultured in 2 different chemical difined custom media. Transcriptional profile were compeared between clones and media.

2022-03-01 | GSE197570 | GEO

iCHO2441

Project description:A synthesized Chinese Hamster Ovary (CHO) cell genome-scale model (GeM) consisting of iCHO2291 (Yeo. et al 2020) coupled with the secretory machinery presented in iCHO2048 (Gutierrez et al. 2020). This model is the most comprehensive CHO GeM to date, boasting the highest number of genes compared to any previous CHO GeM.

2023-03-06 | MODEL2206100001 | BioModels

CHO smallRNA sequencing for microRNA annotation

Project description:MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from CHO cells provide new tools for the discovery of novel miRNAs in this important production system. The current release of the miRNA registry miRBase contains 307 mature miRNAs and 200 precursor sequences. We searched for evolutionary conserved miRNAs in recently published genomic data, derived from hamster and CHO cells, to further extend the list of known miRNAs. With our approach we could identify several dozens novel miRNAs and verify their expression with multiple next-generation sequencing experiments. In addition, a great amount of unexpressed miRNAs could be found, awaiting further confirmation by testing for their transcription in different Chinese hamster tissues. Two batch processes with 6 samples each

2015-03-02 | E-GEOD-59838 | biostudies-arrayexpress

Quantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique

Project description:We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total of eight iTRAQ 8-plex experiments were performed.

2016-07-20 | PXD003462 | Pride

CRISPR screen

Project description:CRISPR screen

| OEX00023089 | NODE

CHO smallRNA sequencing for microRNA annotation

2015-03-02 | GSE59838 | GEO

Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 1

Project description:Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins.

2017-09-04 | PXD006060 | Pride

Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 2

2017-09-04 | PXD006061 | Pride

Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 4

2017-09-04 | PXD006063 | Pride