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Analysis of Escherichia coli adaptation to a sublethal concentration of sodium bisulfite

ABSTRACT: Little is known about cell responses and survival in the presence if reducing agents that could change the redox state of living organisms potentially conferring a negative effect on major metabolic pathways and respiration. As many reducing reagents, in particular sulfur-containing compounds, are widely used as food preservatives, we decided to study effects of sublethal concentrations of reducing reagents on bacterial adaptation. The aim of this study was to analyse and compare the transcriptome profils of untreated and sulfite-treated Escherichia coli cells obtained by employing microarray analysis.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE137882 | GEO | 2020/12/01

REPOSITORIES: GEO

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Analysis of Escherichia coli adaptation to sublethal concentrations of sodium bisulfite

Project description:Little is known about cell survival in the presence of reducing reagents, likewise changing the redox state of living organisms and, therefore potentially conferring a negative effect on respiration and major metabolic pathways. We employed Escherichia coli as a model organism to analyze its responses to sublethal concentrations of sodium sulfite, one of the common food preservatives used for inhibition of microbial growth. We found that partial inhibition of E. coli growth by sodium sulfite was accompanied by profound changes in gene expression. Besides affecting major metabolic pathways, these changes apparently led to reduced production of flagellar proteins, thus potentially impacting cell motility. Concomitantly, E. coli upregulated a number of genes involved in repair of macromolecules and antisense control.

2024-06-20 | E-MTAB-7120 | biostudies-arrayexpress

Analysis of Escherichia coli adaptation to a sublethal concentration of sodium bisulfite

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Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

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Project description:Despite the widespread use of antiseptics such as chlorhexidine digluconate (CHX) in dental practice and oral care, the risks of potential resistance toward antiseptics in oral bacteria have only been highlighted very recently. Since the molecular mechanisms behind antiseptic resistance or adaptation are not entirely clear yet and the bacterial stress response has not been investigated systematically so far, the aim of the present study was to investigate the transcriptomic stress response in Streptococcus mutans after treatment with CHX using RNA sequencing. Planktonic cultures of stationary phase S. mutans were treated with a sublethal dose of CHX (125 µg/mL) for 5 min. After treatment, RNA was extracted, and RNA sequencing was performed on the Illumina NextSeq 500. Differential expressed genes were analyzed and validated by qRT-PCR. The analysis of the differential gene expression following pathway analysis revealed a considerable number of genes and pathways significantly regulated in S. mutans after sublethal treatment with CHX. In summary, expression of 423 genes was up-regulated and 295 genes down-regulated after CHX treatment. Analysis of differentially expressed genes and significantly regulated pathways showed regulation of genes involved in purine nucleotide synthesis, biofilm formation, transport systems and stress responses. In conclusion, these results show an overview of the transcriptomic stress response in S. mutans upon exposure to CHX and give an insight in potential mechanisms that may result in development of resistances.

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