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Analysis of Escherichia coli adaptation to a sublethal concentration of sodium bisulfite

ABSTRACT: Little is known about cell responses and survival in the presence if reducing agents that could change the redox state of living organisms potentially conferring a negative effect on major metabolic pathways and respiration. As many reducing reagents, in particular sulfur-containing compounds, are widely used as food preservatives, we decided to study effects of sublethal concentrations of reducing reagents on bacterial adaptation. The aim of this study was to analyse and compare the transcriptome profils of untreated and sulfite-treated Escherichia coli cells obtained by employing microarray analysis.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE137882 | GEO | 2020/12/01

REPOSITORIES: GEO

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Analysis of Escherichia coli adaptation to sublethal concentrations of sodium bisulfite

Project description:Little is known about cell survival in the presence of reducing reagents, likewise changing the redox state of living organisms and, therefore potentially conferring a negative effect on respiration and major metabolic pathways. We employed Escherichia coli as a model organism to analyze its responses to sublethal concentrations of sodium sulfite, one of the common food preservatives used for inhibition of microbial growth. We found that partial inhibition of E. coli growth by sodium sulfite was accompanied by profound changes in gene expression. Besides affecting major metabolic pathways, these changes apparently led to reduced production of flagellar proteins, thus potentially impacting cell motility. Concomitantly, E. coli upregulated a number of genes involved in repair of macromolecules and antisense control.

2024-06-20 | E-MTAB-7120 | biostudies-arrayexpress

Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

Project description:Purpose: In order to clarify the molecular response mechanism of Tetranychus truncatus to sublethal concentrations of abamectin. Methods: We sequenced the transcriptome of Tetranychus truncatus treated with sublethal concentration of abamectin and control. Results: we provide a comprehensive transcriptome resource for Tetranychus truncatus derived from treating with sublethal concentration abamectin and control. Conclusions: The new genomic resources and data that we present in this study for Tetranychus truncatus will substantially facilitate molecular studies of underlying mechanisms involved in abamectin resistance.

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Analysis of Escherichia coli adaptation to a sublethal concentration of sodium bisulfite

Project description:Analysis of Escherichia coli adaptation to a sublethal concentration of sodium bisulfite

| PRJNA573708 | ENA

Treatment of Candida albicans with sublethal concentration of keanumycin A

Project description:To determine the potential mode of action of the new antifungal compound candidate keanumycin A, the C. albicans wild type SC5314 was exposed to a sublethal dose of the substance. Untreated samples serve as control.

2023-05-31 | E-MTAB-12311 | biostudies-arrayexpress

Transcriptomic stress response in Streptococcus mutans following treatment with a sublethal concentration of chlorhexidine digluconate

Project description:Despite the widespread use of antiseptics such as chlorhexidine digluconate (CHX) in dental practice and oral care, the risks of potential resistance toward antiseptics in oral bacteria have only been highlighted very recently. Since the molecular mechanisms behind antiseptic resistance or adaptation are not entirely clear yet and the bacterial stress response has not been investigated systematically so far, the aim of the present study was to investigate the transcriptomic stress response in Streptococcus mutans after treatment with CHX using RNA sequencing. Planktonic cultures of stationary phase S. mutans were treated with a sublethal dose of CHX (125 µg/mL) for 5 min. After treatment, RNA was extracted, and RNA sequencing was performed on the Illumina NextSeq 500. Differential expressed genes were analyzed and validated by qRT-PCR. The analysis of the differential gene expression following pathway analysis revealed a considerable number of genes and pathways significantly regulated in S. mutans after sublethal treatment with CHX. In summary, expression of 423 genes was up-regulated and 295 genes down-regulated after CHX treatment. Analysis of differentially expressed genes and significantly regulated pathways showed regulation of genes involved in purine nucleotide synthesis, biofilm formation, transport systems and stress responses. In conclusion, these results show an overview of the transcriptomic stress response in S. mutans upon exposure to CHX and give an insight in potential mechanisms that may result in development of resistances.

2022-03-30 | GSE197633 | GEO

A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in L. monocytogenes

Project description:We report the effect of the pediocin-containing supernatant of L. plantarum WHE 92 on the temporal transcriptomic profile of L. monocytogenes EGD Examination of mRNA and sRNA profiles from L. monocytogenes EGD cells exposed to sublethal levels of the pediocin-containing supernatant of L. plantarum WHE 92 at 10, 60 and 180 min of exposure.

2014-01-31 | E-GEOD-51487 | biostudies-arrayexpress

Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

Project description:Transcriptome responses to sublethal concentration of abamectin in Tetranychus truncatus analyzed by deep-sequencing

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Rescue of CD8+ T cell vaccine memory in mice following sublethal g radiation exposure

Project description:In two disparate models, we show that rapid revaccination following sublethal gamma radiation exposure rescues memory CD8+ T cell Responses.

2015-09-01 | GSE64434 | GEO

Spinetoram sublethal exposure stimulates Dengue virus replication in Aedes aegypti by dysregulating oxidative phosphorylation

Project description:Vector-borne diseases are closely linked to the environment by the ecology of the vectors and their hosts. Elucidating these causal relationships is one of the most pressing challenges faced by researchers and public health scientists as increasing anthropogenic alternations in the environment can drive shifts in vector-borne disease dynamics. However, as one of the major environmental stressors, the impact of sublethal exposure of insecticides on vector behavior remains poorly investigated. Here, we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram on Aedes aegypti larvae, the latest generation of the spinosyns that have been suggested as a promising new bioinsecticide for vector control, at the larval stage of Ae. aegypti alters adult performance and susceptibility to dengue virus (DENV) infection. While spinetoram sublethal exposure leads to extended immature development time, these survivors were significantly smaller and exhibited weaker blood-feeding capacity than normal females. More importantly, surviving females presented higher DENV susceptibility than the control group after spinetoram sublethal exposure on larvae. Mechanistically, the transcriptomic analysis showed that inhibition of oxidative phosphorylation (OXPHOS) may function in stimulating DENV production in adult Ae. aegypti. In vitro study revealed that spinetoram induces apoptosis via mitochondrial reactive oxygen species (mtROS)-regulated OXPHOS dysregulation in Aag2 cells, and thereby may stimulate DENV production through the metabolic switch between OXPHOS and glycolysis. Altogether, our data demonstrate that sublethal spinetoram exposure could act as a crucial factor on life traits and vector competence in Ae. aegypti. Given that other insecticides are known to induce mitochondrial dysfunction in mosquito vectors, this research may have wider implications.

2022-09-05 | GSE186219 | GEO

Transcriptomic stress response in Streptococcus mutans following treatment with a sublethal concentration of chlorhexidine digluconate

Project description:Transcriptomic stress response in Streptococcus mutans following treatment with a sublethal concentration of chlorhexidine digluconate

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