Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chemical reprogramming of fibroblasts to photoreceptor like cells [RNA-Seq]

ABSTRACT: Reprogramming of mouse embryonic fibroblasts (MEFs) to photorereceptor like cells by five chemicals.

ORGANISM(S): Mus musculus

PROVIDER: GSE138520 | GEO | 2019/12/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Chemical reprogramming of fibroblasts to photoreceptor like cells [ATAC-Seq]

Project description:Reprogramming of mouse embryonic fibroblasts (MEFs) to photorereceptor like cells by five chemicals.

2019-12-11 | GSE138521 | GEO

Reprogramming MEFs (mouse iPSCs)

Project description:Proteome of reprogramming mouse embryonic fibroblasts (MEFs) with or without Akt activation

2020-07-31 | PXD014856 | JPOST Repository

Chemical reprogramming of fibroblasts to photoreceptor like cells

Project description:Chemical reprogramming of fibroblasts to photoreceptor like cells

| PRJNA576241 | ENA

Chemical reprogramming of human fibroblasts

Project description:Chemical reprogramming of human fibroblasts

| PRJNA509474 | ENA

Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails

Project description:The direct conversion, or trans-differentiation, of non-cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provide promising ways of cardiac regeneration. However, genetic manipulations are still not desirable in real clinical applications. we report the generation of automatically beating cardiomyocyte-like cells from mouse fibroblasts with only chemical cocktails. These chemical-induced cardiomyocyte-like cells (CiCMs) express cardiomyocyte-specific markers, exhibit sarcomeric organization, and possess typical cardiac calcium flux and electrophysiological features. Microarray-bassed gene expression patterns of Mouse embryonic fibroblasts (MEFs), CiCMs, and cardiomyocytes(CMs) indicated a clear transition from dividing MEFs to differentiated cardiomyocyte-like state in CiCM samples. Mouse embryonic fibroblasts were treated with a small-molecule combination CRFVPT (10 μM CHIR99021 (C); 10 μM RepSox (R); 50 μM Forskolin (F); 0.5 mM VPA (V); 5 μM Parnate, (P); 1 μM TTNPB (T)) to induce transdifferentiation to chemical-induced cardiomyocyte-like cells. CiCMs beating clusters were picked at day 24 for analysis. MEFs were isolated from mouse embryos, and CMs were isolated from mouse hearts. Total RNA of MEFs, CiCMs and CMs were extracted and hybridization on Affymetrix microarrays.

2015-08-23 | E-GEOD-69924 | biostudies-arrayexpress

Bulk RNA sequencing of chemical-induced intermediate cells

Project description:This study aims to investigate the individual effect of cocktail VCRTc on the direct reprogramming process.We collected cells as follows: mouse chondrocytes (mchons), mouse embryonic fibroblasts(MEFs), MEFs treated by a chemical cocktail VCRTc for 3 days (3d-VCRTc) and 6 days (6d-VCRTc), and MEFs treated by individual chemical V, C, R, T, ce for 6 days, respectively. Then we did RNA extraction,reverse transcription and library preperation before bulk RNA seq. We uncovered that MEFs treated with chemicals have an upregulation of chondrocytes enriched genes; and a downregulation of MEF enriched genes,indicating that chemical treatment drove MEF toword chondrogenic lineage before the 2nd stage of differentiation.

2018-11-19 | GSE118235 | GEO

Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails

2015-08-23 | GSE69924 | GEO

Chemical reprogramming of fibroblasts to photoreceptor like cells [RNA-Seq]

Project description:Chemical reprogramming of fibroblasts to photoreceptor like cells [RNA-Seq]

| PRJNA576236 | ENA

Chemical reprogramming of fibroblasts to photoreceptor like cells [ATAC-Seq]

Project description:Chemical reprogramming of fibroblasts to photoreceptor like cells [ATAC-Seq]

| PRJNA576238 | ENA

Chemical reprogramming of mouse embryonic and adult fibroblast into endoderm lineage

Project description:We report here an approach to redirect somatic cell fate under chemically defined conditions without transcription factors. We start by converting mouse embryonic fibroblasts (MEFs) to epithelial-like cells (ciELC) with chemicals and growth factors. Subsequent cell fate mapping reveals a robust induction of SOX17 in the resulting ELCs that can be further reprogrammed to endodermal progenitor cells (ciEPC). Interestingly, these cells can self-renew in vitro and further differentiate into albumin-producing hepatocytes that can rescue mice from acute live injury. Our results demonstrate a rational approach to convert MEFs to hepatocytes and suggest that this mechanism-driven approach may be generalized for other cells.

2017-10-04 | GSE104555 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data