Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epigenomes of Hemin exposed trained monocytes [scATAC-seq]

ABSTRACT: Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we apply epigenomic, H3K27ac ChIP-seq, analysis to healthy human monocytes exposed to Hemin, a danger-associated signal, and B-glucan, a yeast sugar, in vitro.

ORGANISM(S): Mus musculus

PROVIDER: GSE141968 | GEO | 2020/02/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Epigenomes of Hemin exposed trained monocytes

Project description:Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we apply epigenomic, H3K27ac ChIP-seq, analysis to healthy human monocytes exposed to Hemin, a danger-associated signal, and B-glucan, a yeast sugar, in vitro.

2020-02-21 | GSE110977 | GEO

Transcriptomes of Hemin exposed trained monocytes

Project description:Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we apply RNA-seq analysis to healthy human monocytes exposed to Hemin, a danger-associated signal, and B-glucan, a yeast sugar, in vitro.

2020-02-21 | GSE110947 | GEO

Epigenomes of Hemin exposed trained monocytes [scATAC-seq]

Project description:Epigenomes of Hemin exposed trained monocytes [scATAC-seq]

| PRJNA595433 | ENA

Epigenomes of Hemin exposed trained monocytes

Project description:Epigenomes of Hemin exposed trained monocytes

| PRJNA435439 | ENA

Transcriptomes and Epigenomes of Hemin exposed trained monocytes

Project description:Transcriptomes and Epigenomes of Hemin exposed trained monocytes

| PRJNA435573 | ENA

Transcriptomes of Hemin exposed trained monocytes

Project description:Transcriptomes of Hemin exposed trained monocytes

| PRJNA435107 | ENA

Epigenomes of oxLDL exposed trained monocytes

Project description:Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components. In this study we apply epigenetic, H3K27ac and H3K4me3 ChIP-seq, analysis to healthy human monocytes exposed to oxidized Low Density Lipoprotein (oxLDL), in vitro.

2019-11-07 | GSE122268 | GEO

Epigenetic programming during monocyte to macrophage differentiation and trained innate immunity

Project description:Monocyte differentiation into macrophages represents one of the cornerstone processes in innate host defense. In addition, immunological imprinting of either tolerance or trained immunity after an initial infection determines the functional fate of innate immune cells and the susceptibility of the host to secondary infections. Here we comprehensively characterize the epigenetic profiles of these functional states relative to healthy adult naM-CM-/ve monocytes. Inflammatory and metabolic pathways are strongly modulated in the derived macrophages, including decreased activation of inflammasome components. The cAMP-dependent signaling pathway is remodeled and adrenergic signaling was functionally implicated in trained innate immunity induction in vivo. Interestingly, M-oM-^AM-"-Glucan trains innate immune cells through extensive remodeling of distal regulatory region-bound histone acetylation, resulting in a sizeable exclusive epigenomic signature. Accordingly, genome-wide transcription factor footprint analysis reveals a specific transcription factor repertoire at trained cell-specific enhancers when recouped with epigenetic data, forming a rich hypothesis generator to manipulate innate immunity. Monocytes were pre-incubated either with cell culture medium (RPMI), M-NM-2-glucan (5M-BM-5g/mL) or with LPS (100ng/mL), for 24 hours in a total volume of 10 mL. After a wash-out, cells were cultured in RPMI supplemented with 10% human pool serum. Monocytes were collected at different time points (0 h and 6 d after treatment) and counted before further treatment for chromatin immunoprecipitation, RNA or DNaseI treatment. Different donor Buffycoats (BC) were used as independent replicates. Replicates were generated for all the profiles including ChIPseq,RNAseq and DNaseIseq.

2014-09-26 | E-GEOD-58310 | biostudies-arrayexpress

Transcriptomes of human trained macrophages before and after Mtb exposure

Project description:Trained Immunity (TRIM) is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to specific microbial or metabolic components. In this study, monocytes were trained with beta-glucan and then re-exposed to Mycobacterium tuberculosis.

2019-12-10 | GSE141656 | GEO

Transcriptomes and Epigenomes of Hemin exposed trained monocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-02-21 | GSE111003 | GEO