Gene expression profile of Skov3 cells cultured with DMSO (Control) or GA-017 in 3D condition
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ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE145538 | GEO | 2021/04/01
REPOSITORIES: GEO
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Project description:Preclinical cancer drug discovery efforts have employed two-dimensional (2D)-cell-based assay models, which fail to forecast in vivo efficacy and contribute to a lower success rates of clinical approval. Three-dimensional (3D) cell culture models are recently expected to bridge the gap between 2D and in vivo models. We have developed a novel 3D culture method that improves the growth of spheroid-forming cancer cells under anchorage-independent condition by leveraging a feature of FP001, a bacteria-derived polysaccharide. Gene microarrays were used to observe the global gene expression in SKOV3 cells cultured with adhesion condition (2D, control) or with low adhesion condition (FP001) and identified distinct classes of up or down-regulated genes.
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Project description:Gene expression profile of Skov3 cells cultured with DMSO (Control) or GA-017 in 3D condition
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