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Single nucleus sequecing data of MCH neurons [scRNA-seq]

ABSTRACT: In order to understand heterogeneity of MCH neurons, by using MCH-Cre dependent ZsGreen (fl/fl) reporter mice, we isolated nuclear from 16 hypothalami of 16 mice at the age of 16-18weeks. Through flow cytometry, we were able to distinguish ZsGreen positive nuclei, and collected puried MCH nuclei in suspension. Those nuclei suspension were subjected to single cell sequence by 10x™ GemCode™ Technology for further single nuleus mRNA analysis and unravel subclusters of MCH neurons

ORGANISM(S): Mus musculus

PROVIDER: GSE146020 | GEO | 2020/05/07

REPOSITORIES: GEO

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