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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP3 RNASeq]

ABSTRACT: In this experiment the relationship between SOX2 levels and WNT-induced gene expression was assayed using RNA-seq during embryonic stem cell differentiation. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE162772 | GEO | 2021/12/31

REPOSITORIES: GEO

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP1_ATACSeq]

Project description:In this experiment the relationship between SOX2 levels and chromatin accessibility was assayed using ATAC-seq during embryonic stem cell differentiation. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP2 ChIPSeq]

Project description:In this experiment the relationship between SOX2 levels and the binding of B-catenin, TCF/LEF factors, and SOX2 to chromatin was assayed using ChIP-seq during embryonic stem cell differentiation and in naive pluripotency. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_ChIPSeq]

Project description:In this experiment the binding of SOX2, B-catenin, and LEF1 was assayed using ChIP-seq following differentiation of WT, T/Bra mutant, and Cdx1,2,4 triple mutant embryonic stem cells to caudal ebiblast progenitors. SOX2 binding in epiblast cells was assayed for comparison.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP4_RNASeq]

Project description:In this experiment the transcriptional changes accompanying differentiation of embryonic stem cell to epiblast-like cells was assayed using RNA-seq.

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Project description:In this experiment the transcriptional changes accompanying differentiation of WT, T/Bra mutant, and Cdx1,2,4 mutant embryonic stem cells to caudal epiblast-like cells was assayed using RNA-seq.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation.

Project description:Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_RNASeq]

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_ChIPSeq]

Project description:Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_ChIPSeq]