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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE162774 | GEO | 2021/12/31

REPOSITORIES: GEO

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP4_RNASeq]

Project description:In this experiment the transcriptional changes accompanying differentiation of embryonic stem cell to epiblast-like cells was assayed using RNA-seq.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP1_ATACSeq]

Project description:In this experiment the relationship between SOX2 levels and chromatin accessibility was assayed using ATAC-seq during embryonic stem cell differentiation. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_ChIPSeq]

Project description:In this experiment the binding of SOX2, B-catenin, and LEF1 was assayed using ChIP-seq following differentiation of WT, T/Bra mutant, and Cdx1,2,4 triple mutant embryonic stem cells to caudal ebiblast progenitors. SOX2 binding in epiblast cells was assayed for comparison.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_RNASeq]

Project description:In this experiment the transcriptional changes accompanying differentiation of WT, T/Bra mutant, and Cdx1,2,4 mutant embryonic stem cells to caudal epiblast-like cells was assayed using RNA-seq.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP3 RNASeq]

Project description:In this experiment the relationship between SOX2 levels and WNT-induced gene expression was assayed using RNA-seq during embryonic stem cell differentiation. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression.

2021-12-31 | GSE162772 | GEO

Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP2 ChIPSeq]

Project description:In this experiment the relationship between SOX2 levels and the binding of B-catenin, TCF/LEF factors, and SOX2 to chromatin was assayed using ChIP-seq during embryonic stem cell differentiation and in naive pluripotency. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression.

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Sox2 levels regulate the chromatin occupancy of WNT mediators in epiblast progenitors responsible for vertebrate body formation.

Project description:WNT signalling has multiple roles. It maintains pluripotency of embryonic stem cells, assigns posterior identity in the epiblast and induces mesodermal tissue. Here we provide evidence that these distinct functions are conducted by the transcription factor SOX2, which adopts different modes of chromatin interaction and regulatory element selection depending on its level of expression. At high levels, SOX2 displaces nucleosomes from regulatory elements with high-affinity SOX2 binding sites, recruiting the WNT effector TCF/β-catenin and maintaining pluripotent gene expression. Reducing SOX2 levels destabilizes pluripotency and reconfigures SOX2/TCF/β-catenin occupancy to caudal epiblast expressed genes. These contain low-affinity SOX2 sites and are co-occupied by T/Bra and CDX. The loss of SOX2 allows WNT-induced mesodermal differentiation. These findings define a role for Sox2 levels in dictating the chromatin occupancy of TCF/β-catenin and reveal how context-specific responses to a signal are configured by the level of a transcription factor.

| S-EPMC9106585 | biostudies-literature

Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation.

Project description:Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation.

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_RNASeq]

Project description:Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_RNASeq]

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Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_ChIPSeq]

Project description:Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation [EXP5_ChIPSeq]

| PRJNA749307 | ENA