Other

Dataset Information

0

Multiplex genome-editing in Arabidopsis thaliana using Mb3Cas12a


ABSTRACT: The use of CRISPR/Cas proteins for the creation of multiplex genome-engineering represents an important avenue for crop improvement, and further improvements for creation of knock-in plant lines via CRISPR-based technologies may enable the high-throughput creation of designer alleles. To circumvent limitations of the commonly used CRISPR/Cas9 system for multiplex genome-engineering, we explored the use of Moraxella bovoculi 3 Cas12a (Mb3Cas12a) for multiplex genome-editing in Arabidopsis thaliana. We identified optimized promoter sequences for driving expression of single transcript multiplex crRNA arrays in A. thaliana, resulting in stable germline transmission of Mb3Cas12a-edited alleles at multiple target sites. By utilizing this system, we demonstrate single-transcript multiplexed genome-engineering using of up to 13 crRNA targets. We further show high target specificity of Mb3Cas12a-based genome-editing via whole-genome sequencing. Taken together, our method provides a simplified platform for efficient multiplex-genome-engineering in plant-based systems.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE162875 | GEO | 2021/09/15

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-04-23 | E-GEOD-44582 | biostudies-arrayexpress
| PRJNA354073 | ENA
| PRJNA818127 | ENA
2022-03-02 | PXD006712 | Pride
| PRJNA601142 | ENA
2016-07-29 | E-GEOD-84534 | biostudies-arrayexpress
2023-09-01 | GSE214548 | GEO
| PRJNA1156607 | ENA
2023-03-20 | GSE223805 | GEO
| PRJNA820998 | ENA