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Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

ABSTRACT: To investigate the function of malpighian tubule in tumor-induced host wasting, we established a wasting model in fly by activation of Yki in adult midgut ISCs, in which the host flies present the sysytmeic organ wasting and metabolic abnormalities.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE240166 | GEO | 2023/08/11

REPOSITORIES: GEO

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Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

Project description:To investigate the function of Malpighian tubules on host wasting, we established a fly model of tumor-induced wasting by activation of an oncogene Yki in adult intestinal stem cells (ISCs) for 8 days to cause gut-tumor formation and systemic organ wasting and metabolic abnormalities. We then performed RNA-seq and gene expression analysis in Malpighian tubules from control and yki3SA tumor-bearing flies

2022-05-29 | GSE204866 | GEO

To explore the change of the gut microbiota in yki3SA tumor-bearing flies by metagenomics sequencing.

Project description:To investigate the effect of gut microbiota on host wasting, we established a wasting model in fly by activation of Yki in adult midgut ISCs, in which the host flies present the sysytmeic organ wasting and metabolic abnormalities.

2022-05-30 | GSE204975 | GEO

Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

Project description:Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

| PRJNA1002389 | ENA

Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation

Project description:Canine heartworm is a widespread and potentially fatal mosquito-borne disease caused by infections with the parasitic nematode, Dirofilaria immitis. We have previously shown that systemic activation of the Toll immune pathway via silencing of the negative regulator Cactus in Aedes aegypti blocks parasite development in the Malpighian tubules, the mosquito renal organ. However, it was not established whether the Malpighian tubules were directly responding to Toll activation or were alternatively responding to upregulated proteins or other changes to the hemolymph driven by other tissues. Distinguishing these possibilities is crucial for developing more precise strategies to block D. immitis while potentially avoiding the fitness cost to the mosquito associated with Cactus silencing. This study defines the transcriptional response of Ae. aegypti Malpighian tubules after systemic Toll activation via intra-thoracic injection of dsCactus and found, like the response of whole mosquitoes, a significant increase in expression of Toll pathway target genes. Additionally, we identified a significant overlap between the transcriptional response of the Malpighian tubules and proteins upregulated in the hemolymph. Our data show that Malpighian tubules are capable of RNAi-mediated gene silencing and directly respond to dsCactus treatment by upregulating canonical Toll pathway targets. Though not definitive, the strong correspondence between the Malpighian tubule transcriptional and the hemolymph proteomic responses provides evidence that the tubules may contribute to mosquito humoral immunity.

2024-08-27 | PXD036225 | Pride

Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

Project description:Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

| PRJNA842589 | ENA

Natural variation affecting allele-specific expression in the Malpighian tubules of Drosophila melanogaster

Project description:We used RNA-seq to investigate natural variation in gene expression in the Malpighian tubules of three inbred Drosophila melanogaster strains and their F1 hybrids. One of the strains was from a population in the species’ ancestral range (Zambia), while the other two were from a more recently derived population (Sweden).

2018-08-29 | GSE103645 | GEO

Transcription profiling of Drosophila pooled Malpighian tubules vs. whole fly

Project description:This is an affymetrix array experiment comparing the transcriptome of the Malpighian tubule (or renal tubule) of 7-day adult Oregon R strain Drosophila melanogaster with matched whole fly samples. It is described in:,Wang, J., Kean, L., Yang, J., Allan, A. K., Davies, S. A., Herzyk, P. and Dow, J. A. T. (2004). Function-informed transcriptome analysis of Drosophila renal tubule. Genome Biol. In press.,There are five tubule samples (each derived from approx 1000 tubules (!)), and 5 matched whole-fly samples. i.e. tubule 2 is dissected from the same vial as WholeFly2.,As the tubule is probably the premier tissue for true physiology in Drosophila, the dataset can usefully be interrogated in conjunction with the detailed physiological understanding of the tissue: see,http://fly.to/tubules

2007-07-06 | E-GEOD-1690 | biostudies-arrayexpress

Expression data from Malpighian tubules of wild type and dVHL +/- Drosophila 5-days old female

Project description:Mutations in VHL tumor suppressor gene are the cause of the familial VHL disease that displays multiple benign and malignant tumors such as clear cell renal cell carcinoma. The VHL gene is similar in Drosophila melanogaster (dVHL) and humans. We used microarrays to compare the gene expression profile between yellow white (used as control) and heterozygous dVHL +/- Malpighian tubes dissected from adult Drosophila female with the aim to identify distinct classes of up-regulated and downregulated genes in a sensitized (haploinsufficient) genetic background.

2019-05-28 | GSE124152 | GEO

Malpigian tubules

Project description:modENCODE_submission_5521 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Most terminally differentiated Drosophila tissues are either polyploid or polytene. Unlike normal chromosomes, where the entire chromosome must be replicated exactly once, polytene chromosomes are often differentially replicated with many regions underreplicated and some overreplicated. We will characterize five different polytene tissues using comparative genomic hybridization (CGH) to identify differentially replicated regions of each chromosome. These studies will also identify tissue specific amplicons, where the replication mediated amplification of specific loci is essential for up-regulation of mRNA levels encoding proteins critical for development. The differential replication of polytene chromosomes in Drosophila will provide a unique opportunity to understand how developmental cues and chromosomal domains influence replication initiation. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CGH. BIOLOGICAL SOURCE 1: Strain: Oregon-R Orr-Weaver(genotype : TBA outcross : TBA description : Wild-type Oregon-R population maintained by Terry Orr-Weaver. made_by : wt population ); Tissue: Malpighian tubules; Developmental Stage: cleavage stage; Genotype: TBA; Sex: Female; BIOLOGICAL SOURCE 2: Strain: Oregon-R Orr-Weaver(genotype : TBA outcross : TBA description : Wild-type Oregon-R population maintained by Terry Orr-Weaver. made_by : wt population ); Tissue: Malpighian tubules; Developmental Stage: cleavage stage; Genotype: TBA; Sex: Unknown; NUMBER OF REPLICATES: 1; EXPERIMENTAL FACTORS: Tissue Malpighian tubules

2012-06-03 | E-GEOD-38439 | biostudies-arrayexpress

Tumor suppressors Sav/Scrib and oncogene Ras regulate stem-cell transformation in adult Drosophila malpighian tubules.

Project description:An increasing body of evidence suggests that tumors might originate from a few transformed cells that share many properties with normal stem cells. However, it remains unclear how normal stem cells are transformed into cancer stem cells (CSCs). Here, we demonstrated that mutations causing the loss of tumor suppressor Salvador (Sav) or Scribble (Scrib) or activation of the oncogene Ras transform normal stem cells into CSCs through a multistep process in the adult Drosophila Malpighian Tubules (MTs). In wild-type MTs, each stem cell generates one self-renewing and one differentiating daughter cell. However, in flies with loss-of-function sav or scrib or gain-of-function Ras mutations, both daughter cells grew and behaved like stem cells, leading to the formation of tumors in MTs. Ras functioned downstream of Sav and Scrib in regulating the stem-cell transformation. The Ras-transformed stem cells exhibited many of the hallmarks of cancer, such as increased proliferation, reduced cell death, and failure to differentiate. We further demonstrated that several signal transduction pathways (including MEK/MAPK, RhoA, PKA, and TOR) mediate Ras' function in the stem-cell transformation. Therefore, we have identified a molecular mechanism that regulates stem-cell transformation, and this finding may lead to strategies for preventing tumor formation in certain organs.

| S-EPMC3391499 | biostudies-literature

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