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RNA-seq analysis of control and MCUR1-depleted HSCs - BioProject

ABSTRACT: We sought to explore the underlying mechanisms by which MCUR1 promotes erythropoiesis. Total RNAs were extracted from cultured human CD34+ HPCs stably expressing sh-MCUR1 or sh-Ctrl under hypoxia (1% O2) following 2 days of EPO stimulation, using TRIZOL Reagent (Invitrogen, CA, USA). RNA sequencing (RNA-seq) was performed using Illumina NovaSeq6000 at Berry Genomics Co. Ltd. (Beijing, China), and a mean of 40 million paired-end reads per sample was obtained.

ORGANISM(S): Homo sapiens

PROVIDER: GSE171094 | GEO | 2021/03/30

REPOSITORIES: GEO

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