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Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE185352 | GEO | 2021/10/07

REPOSITORIES: GEO

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Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Colon]

Project description:MBELN isolated mulberry bark purified by sucrose gradient were orally gavaged to B6 mice for 7 days once in a day at aconcentration of 10 x 10E+09 particle/100 µl. After 7 days, colon tissue were harvested. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after MBELN treatment and their link to gut homeostasis.

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Project description:MBELN isolated mulberry bark purified by sucrose gradient were orally gavaged to B6 mice for 7 days once in a day at aconcentration of 10 x 10E+09 particle/100 µl. After 7 days, colon specific crypt cells were isolated. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after MBELN treatment and their link to gut homeostasis.

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Fecal Exosomes treatment of B6 mice for 14 days

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Project description:Exosomes, small vesicles participating in intercellular communication, have been extensively studied recently; however, the role of edible plant derived exosomes in interspecies communication has not been investigated. Here, we investigate the biological effects of edible plant derived exosome-like nanoparticles (EPDENs) on mammalian cells.In this study, exosome-like nanoparticles from four edible plants were isolated and characterized. We show that these EPDENs contain proteins, lipids, and microRNA. EPDENs are taken up by intestinal macrophages and stem cells. The results generated from EPDEN-transfected macrophages indicate that ginger EPDENs preferentially induce the expression of the antioxidation gene, heme oxygenase-1 and the anti-inflammatory cytokine, IL-10; whereas grapefruit, ginger, and carrot EPDENs promote activation of nuclear factor like (erythroid-derived 2). Furthermore, analysis of the intestines of canonical Wnt-reporter mice, i.e. B6.Cg-Tg(BAT-lacZ)3Picc/J mice, revealed that the numbers of ?-galactosidase(+) (?-Gal) intestinal crypts are increased, suggesting that EPDEN treatment of mice leads to Wnt-mediated activation of the TCF4 transcription machinery in the crypts.The data suggest a role for EPDEN-mediated interspecies communication by inducing expression of genes for anti-inflammation cytokines, antioxidation, and activation of Wnt signaling, which are crucial for maintaining intestinal homeostasis.

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Exosome-like nanoparticles from Mulberry bark prevent DSS-induced colitis via the AhR/COPS8

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