Spatial and Temporal Variation in the Skin Transcriptome of Atopic Dermatitis Assessed by 1.5 mm Mini Punch Biopsies

ABSTRACT: To obtain a detailed molecular understanding of both the temporal and spatial variation in atopic dermatitis (AD), we conducted a longitudinal case-control study, in which we followed a population, the Gentofte AD (GENAD) cohort, of mild-to-moderate AD patients and matched healthy controls for more than a year. By the use of 1.5 mm punch biopsies, we obtained 393 samples from lesional, non-lesional and healthy skin, from multiple anatomic regions at different time points for transcriptomic profiling. After quality control, 339 samples remained for further analysis. We envisage that the application of small biopsies, such as those introduced in this study, combined with omics technologies, will enable future skin research, in which multiple sampling from the same individual will give a more detailed, dynamic picture of how a disease fluctuates in time and space.

ORGANISM(S): Homo sapiens

PROVIDER: GSE193309 | GEO | 2022/04/13

REPOSITORIES: GEO