Project description:Transcription analysis of M. tuberculosis H37Ra devR overexpressing strain (LIX48) versus empty vector control strain (LIX47); H37Rv devR overexpressing strain t (LIX50) versus empty vector control strain (LIX49) to study the effect of DevR overexpression
Project description:human bronchial smooth muscle cells were either infected with empty adenoviral type 5 vector at 40 MOI for 4 days, or Mock infected. Each sample set was then stimulated with a mixture of 10ng/mL IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to RNA harvest. RNA samples were split, and the series consists of 3 slides, 2 of which are dye flipped. Keywords: parallel sample
Project description:RNA from murine mammary C57MG cells transfected with an empty control vector (PLNCx) or an expression vector encoding Wnt-1 was isolated and analyzed on Affymetrix MG-U74Av2 arrays. Sample preparation was done as recommended by the manufacturer. Images were analyzed using Affymetrix Microarray Suite 4.0 software. Comparison of Wnt-1 expressing and control cells revealed Wnt-1 targets. Keywords: ordered
Project description:Agilent 4x44k tobacco micro array of wild type tobacco, empty vector control, and HC-Pro transgenic tobacco plants. Both 1-month old leaves and flowers were analyzed. Three biological replicates were performed of each sample.
Project description:rs08-02_nad. Dark and light. Establish which transcripts are affected by changes in NAD concentration, and whether this depends on light/dark. Three genotypes: Two lines of Arabidopsis transformed to overexpress QPRT, an enzyme involved in NAD synthesis (NadC4.11 and NadC15.3) and a third control line transformed with an empty vector (162). Leaf discs from the two lines of the QPRT+ plants were incubated with quinolinate, the QPRT substrate, to boost leaf NAD. Discs from the empty vector control were incubated on a buffer withour quinolinate. The experiment was performed in the light and the dark. The aim is to compare the QPRT+ quinolinate samples with the empty vector samples, both for the sample series generated in the light and that generated in the dark. Keywords: treated vs untreated comparison
Project description:- Identification of proteins whose expression was affected by lcrX knockout and LcrX-overexpressing strains in Xanthomonas axonopodis pv. glycines str. 8ra - Shotgun proteomic analysis was used - Four strains were used with three biological replicates (total 12 samples). Two samples are the wildtype strains carrying an empty vector: One (XagW+V) for comparison with lcrX-KO strain and the other (XagW_V) for comparison with LcrX-overexpressing strain. The third sample is lcrX-KO strain (Xag2894KO). The last sample is LcrX-overexpressing strain (Xag2894OE).
Project description:RNA-Seq profiling of estrogen-receptor-positive MCF-7 cell lines with different perturbations of non-canonical WNT signaling . The MCF-7 cells were either transfected with an empty vector (pcDNA) or with a ROR2 overexpression construct, in parallel with or without stimulation with recombinant WNT5A. The objective was to find expression-responsive targets of these perturbations as potential drivers of increased cell invasiveness.
Project description:T2 progenies of two transgenic lines overexpressing ERF transcription factor WIN1 were grown on soil in parallel under identical conditions. mRNA was extracted from pooled leaves from multiple plants of each line for the microarray experiement. Plants derived from two lines were used in this experiment: line 13, a "medium" overexpressor, and line 22, a strong overexpressor. Control mRNA was obtained from pooled control plants transformed using an empty binary vector. All plants were first selected on medium containing kanamycin before being transferred to soil. Keywords = wax epidermis transcription factor Arabidopsis Keywords: parallel sample