Dataset Information

Differential gene expression in the posterior joint capsule for immobility in mice

ABSTRACT: To explore the factors involved in arthrofibrosis, we performed a genome-wide screening for mRNA expressed in the posterior capsule after immobilization in mice. A gene ontology term analysis for differentially expressed genes after 2 weeks immobilization indicated that the genes involved in the extracellular region were particularly enriched. A heatmap demonstrated that joint immobilization significantly altered the expressions of genes related to the extracellular region. The MA plot indicated that, compared to naïve mice, 1654 genes were upregulated more than 2-fold, and 985 genes were downregulated more than 2-fold in immobilized mice. The MA plot and heatmap visually revealed dynamic changes in the expression of genes in immobilized posterior capsules compared to those in naïve posterior capsules. Our study represents the first detailed analysis of mouse joint capsule transcriptomes, with biologic replicates, generated by RNA-seq technology. RNA-seq based transcriptome characterization would expedite genetic network analyses and clarify Unresolved pathophysiology of joint contractures.

ORGANISM(S): Mus musculus

PROVIDER: GSE223970 | GEO | 2024/01/01

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Advancements in animal models and cell culture techniques have been invaluable in the elucidation of molecular events and mechanisms regulating muscle atrophy. However, few studies have examined muscle atrophy in humans using modern experimental techniques. The purpose of this study was to examine and validate changes in global gene transcription during immobilization-induced muscle atrophy in humans. Healthy men and women (N=24) were subjected to two weeks of unilateral limb immobilization, with muscle biopsies obtained before, and after 48 hours (48H) and 14 days (14D) of immobilization. Both muscle cross sectional area (~ 5 %) and strength (10-20 %) were significantly reduced in men and women after 14D of immobilization. Micro-array analysis of total RNA extracted from biopsy samples uncovered 575 and 3,128 probes representing multiple genes, which were significantly altered at 48H and 14D, respectively. As a group, genes involved in mitochondrial bioenergetics and carbohydrate metabolism were predominant features at both 48H and 14D, with genes involved in protein synthesis and degradation significantly down-regulated and up-regulated, respectively, at 14D of muscle atrophy. There was also a significant decrease in the protein content of mitochondrial cytochrome c oxidase, and the enzyme activity of cytochrome c oxidase and citrate synthase after 14D of immobilization. Furthermore, protein ubiquitination and oxidative damage were significantly increased by 48H and 14D of immobilization, respectively. These results suggest that transcriptional and post-transcriptional suppression of mitochondrial processes is sustained throughout 14D of immobilization, while protein ubiquitination plays an early but transient role in the progression of immobilization-induced muscle atrophy in humans. 72 samples taken from quadriceps of healthy ambulatory young men and women. Samples were taken at 3 timepoints throughout a 14 day period, the initial sample was taken at time 0 (PRECAST), then the limb was immobilized via a brace, and the 2nd sample was taken at 48 hours (CAST02D). The third sample was taken following 14 days of immobilization (CAST14D).

Dataset Information

Differential gene expression in the posterior joint capsule for immobility in mice

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets