The role of SARS-CoV-2 nucleocapsid protein in effecting immune cells and insights on its molecular mechanisms
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ABSTRACT: The study aims were to explore the immune regulatory function of SARA-CoV-2 Nucleocapsid (N protein) and the related mechanisms. In a series of protein activity experiments, the SARA-CoV-2 Nucleocapsid showed the proliferation effect on three immune cell lines (Raw264.7, Jurkat and Raji) in a dose-dependent manner. N protein(10μg/mL) could significantly change cell cycle progressing of these three immune cell lines and could promote Raw264.7 cells quickly entering G2/M phase from the S phase to achieve rapid growth. Additionally, N protein((10μg/mL) also could stimulate Raw264.7 cells to secrete a large number of proinflammatory factors such as TNFα, IL6 and IL10. The RNA sequencing analysis indicated that N protein changed the expression of some certain genes significantly in immune related function and four important signaling pathways including JAK-STAT signaling pathway, TNF signaling pathway, NF-kappa B signaling pathway and MAPK signaling pathway, which suggested that N protein could not only regulate the expression of genes involved in the process of resisting virus infection in macrophages of immune system, but also change the cellular signal processing.
ORGANISM(S): Mus musculus
PROVIDER: GSE236800 | GEO | 2023/07/12
REPOSITORIES: GEO
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