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Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

ABSTRACT: Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

PROVIDER: PRJNA842589 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19415691_1.fastq.gz	Fastqsanger.gz
	SRR19415691_2.fastq.gz	Fastqsanger.gz
	SRR19415692_1.fastq.gz	Fastqsanger.gz
	SRR19415692_2.fastq.gz	Fastqsanger.gz
	SRR19415693_1.fastq.gz	Fastqsanger.gz

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Malpighian tubules (MT) gene expression between control and yki3SA gut-tumor-bearing flies.

Project description:To investigate the function of Malpighian tubules on host wasting, we established a fly model of tumor-induced wasting by activation of an oncogene Yki in adult intestinal stem cells (ISCs) for 8 days to cause gut-tumor formation and systemic organ wasting and metabolic abnormalities. We then performed RNA-seq and gene expression analysis in Malpighian tubules from control and yki3SA tumor-bearing flies

2022-05-29 | GSE204866 | GEO

Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

Project description:Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

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Difference of gene expression in Malpighian tubules (MTs) betweeen control, "yki3SA" tumor, and "yki3SA+ITP-i" tumor-bearing flies

Project description:To investigate the function of malpighian tubule in tumor-induced host wasting, we established a wasting model in fly by activation of Yki in adult midgut ISCs, in which the host flies present the sysytmeic organ wasting and metabolic abnormalities.

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Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation

Project description:Canine heartworm is a widespread and potentially fatal mosquito-borne disease caused by infections with the parasitic nematode, Dirofilaria immitis. We have previously shown that systemic activation of the Toll immune pathway via silencing of the negative regulator Cactus in Aedes aegypti blocks parasite development in the Malpighian tubules, the mosquito renal organ. However, it was not established whether the Malpighian tubules were directly responding to Toll activation or were alternatively responding to upregulated proteins or other changes to the hemolymph driven by other tissues. Distinguishing these possibilities is crucial for developing more precise strategies to block D. immitis while potentially avoiding the fitness cost to the mosquito associated with Cactus silencing. This study defines the transcriptional response of Ae. aegypti Malpighian tubules after systemic Toll activation via intra-thoracic injection of dsCactus and found, like the response of whole mosquitoes, a significant increase in expression of Toll pathway target genes. Additionally, we identified a significant overlap between the transcriptional response of the Malpighian tubules and proteins upregulated in the hemolymph. Our data show that Malpighian tubules are capable of RNAi-mediated gene silencing and directly respond to dsCactus treatment by upregulating canonical Toll pathway targets. Though not definitive, the strong correspondence between the Malpighian tubule transcriptional and the hemolymph proteomic responses provides evidence that the tubules may contribute to mosquito humoral immunity.

2024-08-27 | PXD036225 | Pride

Malpigian tubules

Project description:modENCODE_submission_5521 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Most terminally differentiated Drosophila tissues are either polyploid or polytene. Unlike normal chromosomes, where the entire chromosome must be replicated exactly once, polytene chromosomes are often differentially replicated with many regions underreplicated and some overreplicated. We will characterize five different polytene tissues using comparative genomic hybridization (CGH) to identify differentially replicated regions of each chromosome. These studies will also identify tissue specific amplicons, where the replication mediated amplification of specific loci is essential for up-regulation of mRNA levels encoding proteins critical for development. The differential replication of polytene chromosomes in Drosophila will provide a unique opportunity to understand how developmental cues and chromosomal domains influence replication initiation. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CGH. BIOLOGICAL SOURCE 1: Strain: Oregon-R Orr-Weaver(genotype : TBA outcross : TBA description : Wild-type Oregon-R population maintained by Terry Orr-Weaver. made_by : wt population ); Tissue: Malpighian tubules; Developmental Stage: cleavage stage; Genotype: TBA; Sex: Female; BIOLOGICAL SOURCE 2: Strain: Oregon-R Orr-Weaver(genotype : TBA outcross : TBA description : Wild-type Oregon-R population maintained by Terry Orr-Weaver. made_by : wt population ); Tissue: Malpighian tubules; Developmental Stage: cleavage stage; Genotype: TBA; Sex: Unknown; NUMBER OF REPLICATES: 1; EXPERIMENTAL FACTORS: Tissue Malpighian tubules

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Natural variation affecting allele-specific expression in the Malpighian tubules of Drosophila melanogaster

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Population- and sex-biased gene expression in the excretion organs of Drosophila melanogaster

Project description:We used RNA-seq to investigate gene expression variation in Malpighian tubules, which have a function analogous to that of human kidneys. In order to characterize population differentiation, we sequenced the Malpighian tubule transcriptomes of flies derived from two populations, one from sub-Saharan Africa (Zimbabwe) and one from Europe (the Netherlands). Males and females were examined separately. Overall, we found a high amount of differential expression between sexes (2,308 genes) and populations (2,474 genes). Although most of the differentially expressed genes were consistent between sexes and populations, there were 615 genes showed sex-biased expression in only one population and 557 genes showed population-biased expression in only one sex. mRNA expression profiles of Drosophila melanogaster Malpighian tubules from adult males and females from a European and an African population (2 biological replicates per sex and population)

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Transcription profiling of Drosophila pooled Malpighian tubules vs. whole fly

Project description:This is an affymetrix array experiment comparing the transcriptome of the Malpighian tubule (or renal tubule) of 7-day adult Oregon R strain Drosophila melanogaster with matched whole fly samples. It is described in:,Wang, J., Kean, L., Yang, J., Allan, A. K., Davies, S. A., Herzyk, P. and Dow, J. A. T. (2004). Function-informed transcriptome analysis of Drosophila renal tubule. Genome Biol. In press.,There are five tubule samples (each derived from approx 1000 tubules (!)), and 5 matched whole-fly samples. i.e. tubule 2 is dissected from the same vial as WholeFly2.,As the tubule is probably the premier tissue for true physiology in Drosophila, the dataset can usefully be interrogated in conjunction with the detailed physiological understanding of the tissue: see,http://fly.to/tubules

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2022-02-17 | PXD026817 | Pride

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