Coordination between transcriptional orientation and nucleoprotein complexes generate chromatin configuration of IgH locus [ChIP-Seq]
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ABSTRACT: In this study we combined epigenetic, transcriptional and recombinational assays of wild type and mutated Igh alleles to uncover structure function relationships between sequences that configure the locus for the first step of gene assembly.
Project description:In this study we combined epigenetic, transcriptional and recombinational assays of wild type and mutated Igh alleles to uncover structure function relationships between sequences that configure the locus for the first step of gene assembly.
Project description:In this study we combined epigenetic, transcriptional and recombinational assays of wild type and mutated Igh alleles to uncover structure function relationships between sequences that configure the locus for the first step of gene assembly.
Project description:In this study we combined epigenetic, transcriptional and recombinational assays of wild type and mutated Igh alleles to uncover structure function relationships between sequences that configure the locus for the first step of gene assembly.
Project description:Coordination between transcriptional orientation and nucleoprotein complexes generate chromatin configuration of IgH locus [HiChIP]
Project description:Coordination between transcriptional orientation and nucleoprotein complexes generate chromatin configuration of IgH locus [ChIP-Seq]
Project description:Coordination between transcriptional orientation and nucleoprotein complexes generate chromatin configuration of IgH locus [Capture Hi-C]
Project description:Coordination between transcriptional orientation and nucleoprotein complexes generate chromatin configuration of IgH locus [Directional RNA-Seq]
Project description:Our studies have revealed that a large class of CTCF binding sites – namely the upstream sites – conform neither to a conformational nor a local recombinase activating role. Their conserved spatial distances upstream of V gene segments suggests a possible role in insulating V gene segments from neighboring V gene segments. In any case, understanding the sequence determinants of CTCF binding to the murine IgH locus should facilitate future studies evaluating how IgH locus accessibility regulates CTCF binding as well as the functions that CTCF plays in regulating the recombinational accessibility of VH gene segments during B cell development.