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Effect of PTEN treatment on the subcutaneous tumor formation model of PTEN knockout B16F10 cells in C57BL/6J mice

ABSTRACT: PTEN treatment can affect the tumor growth of PTEN knockout B16-F10 cells in C57BL/6J mice，We hope to analyze the changes that occur in various cells in tumors through single-cell RNA sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE249052 | GEO | 2024/07/24

REPOSITORIES: GEO

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Transcriptomics of PTEN knockout B16-F10 cells in C57BL/6J mice model treated with PTEN

Project description:extracellular PTEN treatment did not affect the growth of PTEN knockout B16-F10 cells cultured in vitro. , To investigate whether extracellular PTEN act on the tumor microenvironment to exert a tumor-suppressive role in vivo，molecular changes caused by PTEN treatment inside the B16-F10-PTEN tumors were monitored by RNA sequencing.

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Transcriptomics of PTEN knockout B16-F10 cells in C57BL/6J mice model treated with PTEN

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Effect of PTEN treatment on the subcutaneous tumor formation model of PTEN knockout B16F10 cells in C57BL/6J mice

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Impact of sevoflurane priming on B16F10 transcriptome after in vivo growth

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Project description:We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection. There were 6 groups or conditions (CCR5 - 6 hours, WT - 6 hours, CCR5 - 24 hours, WT - 24 hours, CCR5 - 48 hours, WT - 48 hour); each group had 4 to 5 mice. All of the mice were injected with 100,000 B16 F10 melanoma cells by tail vein. At the designated time, the mice were anesthetized with Avertin and their lungs were perfused with PBS. After this, the lungs were harvested and snap frozen in liquid nitrogen. They were kept at minus 80 degrees C until processing. mRNA was extracted as described below and equivalent amounts of mRNA (by weight) was pooled by group.

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